SHIP和Caspase-3 mRNA在骨髓增生异常综合征患者骨髓组织中的表达及其临床意义

［摘 要］ 目的：检测骨髓增生异常综合征（MDS）患者骨髓组织中SHIP、Caspase-3 mRNA的表达，探讨其表达与MDS发病及向白血病转化过程的关系。方法：根据国际预后评分系统（IPSS）选择62例MDS患者，其中低危（low-risk）组20例、中危1（Int-1）组12例、中危2（Int-2）组13例和高危（high-risk）组17例，采用RT-PCR法检测62例MDS患者及30例健康正常者(对照组)骨髓单个核细胞(BMNC)中SHIP mRNA及Caspase-3 mRNA的表达水平，并分析两者之间的关系。 结果：MDS低危组和中危1组BMNC中SHIP mRNA表达率（100%，100%）及表达水平(4.467±2.899,4.529±1.975)分别与对照组（100%，4.623±2.979）比较差异均无统计学意义（P>0.05）；在中危2组和高危组中，BMNC中SHIP表达率（92%，88%）下降，表达水平(2.124±1.880,2.574±1.807)低于对照组（P<0.05）。Pearson相关分析，MDS患者BMNC中SHIP mRNA与Caspase-3 mRNA表达水平呈正相关关系（r=0.714，P<0.05）。随骨髓原始细胞比例升高，SHIP mRNA表达水平降低(F=3.7,P<0.05) ，不同性别（t=0.940，P>0.05）、不同年龄（t=1.419，P>0.05）患者SHIP mRNA表达水平差异无统计学意义。 结论：SHIP在高危MDS患者BMNC中呈低表达或不表达，其表达水平可以作为判定MDS预后的一个指标。

Expressions of SHIP and Caspase-3 mRNA in bone marrow tissue of patients with myelodysplastic syndrome and their clinical significances

Objective To detect the expressions of SHIP mRNA and Caspase-3 mRNA in bone marrow tissue of the patients with myelodysplastic syndrome(MDS) and to discuss the relationship between SHIP mRNA and Caspase-3 mRNA expressions and the occurrence and development of MDS.Methods 20 low-risk MDS cases(low-risk group),12 Int-1 MDS cases(Int-1 group),13 Int-2 MDS cases(Int-2 group),and 17 high-risk MDS cases(high-risk group) were selected according to International Prognostic Scoring Syterm(IPSS).30 healthy volunteers were regared as control group.The expression levels of SHIP mRNA and Caspase-3 mRNA in bone marrow mononuclear cells(BMNC) in various groups were detected by RT-PCR method,and the relationship between them was analyzed.Results The positive rates of SHIP mRNA were 100% and 100% and its expression levels were 4.467±2.899 and 4.529±1.975 in low-risk group and Int-1 group,respectively,while they were 100% and 4.623±2.979 in control group,so there were no significant differences between low-risk group,Int-1 group and control group(P>0.05).The positive rates of SHIP mRNA were 90% and 88% and the expression levels were 2.124±1.880 and 2.574±1.807 in Int-2 group and high-risk group,respectively,so there were significantly differences compared with control group(P<0.05).The Pearson correlation analysis results showed that the positive correlations were found between the level of SHIP mRNA and the level of Caspase-3 mRNA in MDS patients(r=0.714,P>0.05).The expression level of SHIP mRNA was decreased with the increasing of premitive cells(F=3.7,P<0.05).There were no significant differences of SHIP mRNA expression levels of patients between different genders(t=0.940,P>0.05) and different ages(t=1.417,P>0.05).Conclusion The expression level of SHIP is lower or the SHIP doesn't express in BMNC of the high-risk MDS patients,and its expression level can be used as an indicator to judge the prognosis of MDS.