葛根素对急性乙醇中毒大鼠脑内阿片肽和多巴胺的调控作用

目的： 检测葛根素对急性乙醇中毒大鼠皮层、小脑、海马和纹状体内阿片肽和多巴胺（DA）表达水平的调控作用，为临床防治乙醇中毒和开发中药葛根提供依据。方法： 雄性SD大鼠24只随机分为对照组、乙醇中毒组和葛根素组，每组8只，采用放射免疫法检测各组大鼠皮层、小脑、海马和纹状体内β-内啡肽（β-EP）、强啡肽（DnyA）和亮脑啡肽（L-EK）表达水平，采用高效液相色谱法检测各组大鼠各脑区DA、多巴克（DOPAC）和高香草酸（HVA）的表达水平，并计算（DOPAC+HVA）/DA的比值。结果：与对照组比较，乙醇中毒组和葛根素组大鼠皮层内β-EP、DnyA和L-EK表达水平降低(P<0.01)，皮层、小脑、海马和纹状体内 DA、DOPAC和HVA表达水平均升高 (P<0.01)；与乙醇中毒组比较，葛根素组大鼠皮层内β-EP、DnyA和L-EK表达水平升高(P<0.05,P<0.01)，DA(P<0.01)、DOPAC(P<0.01)和HVA表达水平均降低，小脑、海马和纹状体内所有指标表达水平均降低(P<0.05,P<0.01)，皮层、小脑和海马内（DOPAC+HVA）/DA 比值升高，纹状体内该比值降低（P>0.05）。结论：葛根素对急性乙醇中毒造成的脑组织损伤有保护作用，其机制可能与葛根素对脑内阿片肽和DA的调控有关。

Regulation of puerarin on endorphin and dopamine in brain of rats with acute alcoholism

Objective To investigate the regulation of puerarin on the expression levels of endorphin and dopamine(DA) of cortex,cerebellum,hippocampus,and striatum of the rats with acute alcoholism and to provide basis for clinical treatment of alcoholism and the exploitation of traditional Chinese herb pueraria.Methods 24 male SD rats were randomly divided into control group,alcoholism group and puerarin group,and there were 8 rats in each group.The expression levels of β-endorphin(β-EP),dynorphin(DnyA) and Leu-enkephalin(L-EK) in cortex,cerebellum,hippocampus and striatum were detected by radioimmunoassay,and the expression levels of DA,dihydroxy-phenyl acetic acid(DOPAC) and homovanillic acid(HVA) of the rats in various groups were detected by high performance liquid chromatography(HPLC) method.The ratio of(DOPAC+HVA)/DA was calculated.Results Compared with control group,the exrpession levels of β-EP,DnyA and L-EK in cortex of the rats in alcoholism group and puerarin group were decreased(P<0.01).The expression levels of DA,DOPAC and HVA in various brain regions were increased(P<0.01).Compared with alcoholism group,and the expression levels of β-EP,DnyA and L-EK in rat cortex in puerarin group were increased(P<0.05,P<0.01),and the expression levels of DA(P<0.01),DOPAC(P<0.01) and HVA in cortex and all the indexes mentioned above in the cerebellum,hippocampus and striatum were decreased(P<0.05,P<0.01),and the ratios of(DOPAC+HVA)/DA in cortex,cerebellum and hippocampus were increased,but it was decreased in striatum(P>0.05).Conclusion Puerarin has protective effect on rat brain damage induced by acute alcoholism and the mechanism may be related to the regulation of puerarin on endorphin and DA on the rat brain.