A double-stranded RNA as the genome of a potential virus infecting <Emphasis Type="Italic">Vicia faba</Emphasis> |
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Authors: | Weixia Liu and Jishuang Chen |
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Institution: | (1) Institute of Bioengineering, Zhejiang Sci-Tech University, Road No.2, Xiasha, Hangzhou, Zhejiang, 310018, People’s Republic of China;(2) Institute of Bioresource Engineering, Nanjing University of Technology, NO.5 Xinmofan Road, Nanjing, Jiangsu, 210009, People’s Republic of China |
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Abstract: | Preparations of double-stranded (ds) RNAs extracted from naturally infected Vicia faba Linn. growing in Hangzhou, Zhejiang Province, Eastern China displayed 3 dominant bands (FaR1, FaR2, and FaR3). FaR2 and FaR3
were found to be identical to the genomic dsRNAs of a recently reported Vicia cryptic virus (VCV). The positive strand of
FaR1 contained two large open reading frames (ORFs), ORF1 and ORF2. The putative proteins encoded by these ORFs were found
to have certain similarities to the putative capsid protein ABO36237] and RNA-dependent RNA polymerase ABC96788], respectively,
of Tomato yellow stunt virus. Thus, FaR1 may represent the genome of a new dsRNA virus, which we have named Vicia cryptic
virus M.
The GenBank Accession numbers of the sequences reported in this paper are EU605883, EU605884, and EU371896. |
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Keywords: | Cryptic virus Double-stranded RNA Sequence analysis Virus genome Vicia faba |
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