PTEN、p27蛋白在胃癌组织中的表达及其相关性

 目的　研究胃癌组织中PTEN和p27蛋白的表达及二者间的相关性。方法　应用免疫组织化学方法检测49例胃癌组织、49例癌旁组织和13例正常胃黏膜中PTEN和p27蛋白的表达情况。结果　胃癌组织中PTEN和p27蛋白的阳性表达率分别为61.22 %（30／49）和57.14 %（28／49），较癌旁组[91.84 %（45／49）、93.88 %（46／49）]和正常组织[100 %（13／13）、100 %（13／13）]明显降低（均P＜0.05）。两者在胃癌中的表达有相关性（r＝0.580，P＝0.000），且均与肿瘤的分化程度、淋巴结转移和PTNM分期有关。结论　PTEN和p27基因的异常改变可能参与胃黏膜细胞的恶性转化过程，且两者之间存在相关性。联合检测两者表达水平可作为评价胃癌病理生物学行为的客观指标之一。

Expression and correlation of PTEN and p27 protein in gastric carcinoma

Objective To investigate the correlation between PTEN and p27 expression in gastric carcinoma. Methods Immunohistochemistry was applied to examine the expression of PTEN and p27 in 49 cases of human gastric carcinoma,49 cases of tumor-side ward mucosa and 13 cases of normal gastric mucosa. Results Positive expressive rates of PTEN and p27 protein in colorectal carcinoma were 61.22 % （30/49） and 57.14 % （28/49）, which were lower than those in tumor-side ward mucosa [91.84 %（45/49） and 93.88% （46/49）] and normal eoloreetal mucosa [100 %（13/13） and 100 %（13/13）]（P〈0.05）. The expression of PTEN and p27 in gastric carcinoma correlated with each other （r=0.580,P=0.005）, and which were associated with degree of differentiation of carcinoma, PTNM stage and lymph node metastasis of the tumors. Conclusion The abnormal expression of PTEN and p27 participate the malignant conversion process of the gastric mucosa cell and the expression levels of PTEN and p27 protein may be used to evaluate the pathologic biology behavior of gastric carcinoma.