FoxA 2在人类非小细胞肺癌组织中的表达及其临床意义

 目的　探讨非小细胞肺癌（NSCLC）组织中肝细胞核因子3β（FoxA 2）的表达情况，及与临床病理因素的关系。方法　分别采用免疫组织化学法和反转录聚合酶链反应，检测80例NSCLC组织及癌旁正常肺组织中FoxA 2蛋白及mRNA的表达，并分析其与患者性别、临床病理类型、吸烟、临床分期、淋巴结转移的关系。结果　FoxA 2蛋白和mRNA在腺癌、鳞状细胞癌、腺鳞癌组织中的表达均低于癌旁正常肺组织（蛋白表达比较，t值分别为187.51、186.43、178.24；mRNA表达比较，t值分别为236.70、260.13、126.27，均 P＜0.05），不同病理类型间FoxA 2蛋白和mRNA的表达差异无统计学意义（F值分别为1.86、1.69，P＞0.05），在低分化组、Ⅲ期及有淋巴结转移患者的肺组织中的表达显著低于相应的高分化组、Ⅰ～Ⅱ期及无淋巴结转移的患者（蛋白比较t值分别为4.31、3.92、3.41；mRNA比较t值分别为5.44、3.28、5.07，均P＜0.05）。结论　FoxA 2可能对人类NSCLC的形成起抑制作用，并参与了肿瘤的分化、淋巴结转移过程，且与肺癌的临床分期相关。

Expression and clinical significance of FoxA 2 in human non-small cell lung cancer

Objective To investigate the expression and clinical significance of FoxA 2（hepatocyte nuclear factor 3β） in human non-small cell lung cancer （NSCLC） tissues and adjacent normal lung tissue. Methods The expressions of FoxA 2 protein in 80 cases with NSCLC tissues and adjacent normal lung tissue were tested by immunohistochemistry, the expressions of mRNA was detected by RT-PCR. Results The expressions of FoxA 2 both on protein and mRNA in NSCLC lung tissue were significantly less than those of adjacent normal lung tissue （protein expression comparison t value was 187.51, 186.43, 178.24 respectively; mRNA expression comparison t value was 236.70, 260.13, 126.27, respeetively,P 〈 0.05）. However there was no difference on histopathological variability. F value equal to 1.86 and 1.69,P 〉 0.05）. In low differentiated group, the group of Ⅲ stage, with lymph node metastasis, the expression of FoxA 2 protein and mRNA was less than that in the group of high differentiated, the group of Ⅰ -Ⅱstage, the group of non lymph node metastasis （pretein expression t values were 4.31, 3.92,341,mRNA expression t values were 5.44, 3.28, 5.07, P〈0.05）. Conclusion FoxA 2 might inhibit progression of human NSCLC. It may participate in the process of tumor tissue differentiation and lymph node metastasis in NSCLC.