巢蛋白和Ki-67在恶性外周神经鞘膜瘤中的表达及其意义

 目的　探讨恶性外周神经鞘膜瘤（MPNST）中巢蛋白和细胞增殖核抗原Ki-67的表达情况及其意义。方法　采用免疫组织化学SP法检测42例MPNST中巢蛋白和Ki-67的蛋白表达。24例外周神经良性肿瘤作为对照。结果　MPNST中巢蛋白阳性表达率为95.2 %（40／42），其中巢蛋白强阳性表达率为40.5 %（17／42），明显高于外周神经良性肿瘤的4.2 %（1／24），差异有统计学意义（χ2＝8.403，P＝0.004）；MPNST中Ki-67标记指数介于1 %～70 %之间，其中＞3 %的占64.3 %（27／42），24例外周神经良性肿瘤中Ki-67标记指数均＜3 %，两者比较差异有统计学意义（χ2＝23.518，P＝0.000）。结论　联合检测巢蛋白和Ki-67免疫标志物有助于MPNST的诊断。

Expression of nestin and Ki-67 in malignant peripheral nerve sheath tumors and its significance

Objective To explore the expression of nestin and Ki-67 in malignant peripheral nerve sheath tumors （MPNST） and its significance in the differential diagnosis. Methods Immunohistochemical technique （SP） was used to detect the expression of nestin and Ki-67 in 42 cases of MPNST and 24 cases of benign peripheral nerve tumor. Results Total expression of nestin was found in 95.2 %（40 / 42） of MPNST. Strong expression of nestin was detected more frequent in MPNST compared to benign peripheral nerve tumors [40.5 %（17/42） versus 4,2 %（1/24）, X2 =8.403,P =0.004]. Ki-67 labeling index in MPNST varied from 1%- 70 %.However, greater than 3 % labeling index of Ki-67 staining was observed in 64.3 %（27 / 42） of MPNST while none of the 24 benign tumors had nuclear staining exceeding 3 %. The higher Ki-67 labeling index showed significant differences between the two groups 0（z = 23.518,P =0.000）. Conclusion Nestin and Ki- 67 are useful markers in distinguishing MPNST from benign tumors.