脂筏对子宫颈癌细胞生长的影响

 目的　探讨脂筏对子宫颈癌细胞生长的影响并初步探讨其作用机制。方法　将HeLa细胞系分为不处理对照组、脂筏干扰剂组及NADPH氧化酶抑制剂组，四甲基偶氮唑蓝（MTT）法测定各组培养24 h后的细胞存活率，Western blot法检测各组细胞内缺氧诱导因子1α（HIF-1α）蛋白相对含量。结果　与对照组相比脂筏干扰剂组细胞的生长速度明显减慢（0.612±0.051 与 0.984±0.034），NADPH氧化酶抑制剂组显示了类似的效应（0.591±0.074与0.984±0.034），差异有统计学意义（t＝4.062,P＜0.05）。与对照组相比脂筏干扰剂组及NADPH氧化酶抑制剂组HIF-1α的表达量也明显降低（1.79±0.14与 2.56±0.22；1.54±0.12 与 2.56±0.22），差异有统计学意义（t＝2.423,P＜0.05）。结论　脂筏可能通过NADPH氧化酶激活途径激活HIF-1α及其下游原癌基因促进子宫颈癌细胞的生长，脂筏干扰剂及NADPH氧化酶抑制剂可能成为子宫颈癌药物治疗新的研究方向。

Effect of lipid raft on the growth of cervical cancer cells

Objective To explore the effect of lipid raft on cervical cancer cell growth and its mechanisms. Methods HeLa cells in logarithmic phase were divided into three groups including control group, lipid raft interference agent group, and NADPH oxidase inhibitors group. Cells were treated with fresh medium, 3 umol/L Apocynin and 1 mmol/L M-beta CD, respectively, for 24 h.Cell survival rate was detected using the MTT method, and the HIF-1a level was examined by Western-blot. Results Cell growths of the lipid raft interference agent group and NADPH oxidase inhibitors group were significantly slower than control group, （0.612±0.051 vs 0.984±0.034, 0.591±0.074 vs 0.984±0.034, t=4.062, P〈0.05）. HIF-1a expression in the lipid raft interference agent group and NADPH oxidase inhibitors group was also significantly reduced compared with control group （1.79±0.14 vs 2.56±0.22 and 1.54±0.12 vs 2.56±0.22） and the difference was significant （t=2.423,P〈0.05）. Conclusion Lipid raft-NADPH oxidase pathway may activate HIF-1a and downstream protocarcinogenic gene expression to promote the growth of cervical cancer cells. The inhibitors of lipid raft and NADPH oxidase may become a new research direction for cervical cancer chemotherapy.