卵巢浆液性肿瘤中Survivin和Caspase-3蛋白表达的相关性及其临床意义义

 目的　分析卵巢浆液性肿瘤中Survivin和Caspase-3 蛋白的表达水平及相互关系，探讨二者在肿瘤发生、发展中的作用。方法　应用流式细胞术定量检测43例卵巢组织标本中Survivin和Caspase-3蛋白的表达。结果　卵巢浆液性囊腺癌中Survivin蛋白表达量高于正常组织和卵巢浆液性囊腺瘤，三者比较差异有统计学意义（F＝19.80；P＝0.00）。Survivin蛋白在早期卵巢浆液性囊腺癌中的表达量明显低于晚期，差异有统计学意义（t＝2.882，P＝0.009）。Survivin蛋白在高中分化和低分化的卵巢浆液性囊腺癌中的表达量比较差异无统计学意义（t＝2.001，P＝0.055）。Survivin蛋白在卵巢浆液性囊腺癌有无淋巴结转移组表达量的比较差异无统计学意义（t＝0.318，P＝0.912）。Caspase-3蛋白在卵巢浆液性囊腺癌中的表达量明显低于卵巢浆液性囊腺瘤和正常卵巢组织，三者比较差异有统计学意义（F =16.32，P＝0.00），且与卵巢浆液性囊腺癌的临床分期、组织学分级、淋巴结转移无关（P＞0.05）。 Survivin与Caspase-3蛋白的表达量呈负相关（r＝-0.413, P＝0.029）。结论　Survivin和Caspase-3在卵巢浆液性肿瘤中的表达呈负相关，二者联合检测或可作为卵巢浆液性肿瘤辅助诊断及评价肿瘤恶性程度的参考指标。

Relationship of Survivin and Caspase-3 protein in ovarian serous tumors and their clinical signifficance

Objective To investgate the expression of Survivin and Caspase-3 in primary ovarian carcinoma, and to explore their relationship. Methods The expression of Survivin and Caspase-3 was detected by flow cytometry （FCM） Results FCM showed that the expression of Survivin in ovarian serous carcinoma was higher than that in serous cystadenoma and normal ovarian tissues （F=19.80;P=0.00） and correlated with the clinical stage（t=-2.882, P=-0.009）. The expression of Caspase-3 in ovarian serous carcinoma was higher than that in serous cystadenoma and normal ovarian tissues （F=16.32,P=0.00）and has no correlativity with clinical stage, histological grade, and lymph metastasis （P〉0.05）. Survivin expression was negatively correlated with Caspase-3 expression in ovarian serous tumors （r=-0.43, P=0.029）. Conclusion Survivin was negatively correlated with Caspase-3 in ovarian serous tumors.Combination of detecting the two protein expressions might be valuable in diagnosis and determining the malignant degree in ovarian serous carcinoma.