氟接触人群血清中硬骨素的表达及其与氟性骨损伤的关系

目的]观察氟接触人群血清中人硬骨素（sclerostin,SOST）的水平,分析其与碱性磷酸酶（alkalinephosphatase,ALP）及氟性骨损伤的相关性,探讨SOST在氟性骨损伤发生中的作用及其机制。方法]采用问卷调查氟接触人群的一般情况、既往史及现患疾病情况,采集静脉血检测SOST、ALP、血氟等指标,并对其进行前臂正位X线检查,采用SPSS 18.0统计软件对数据进行统计学分析。结果]高氟组SOST为（4.806±0.525）μg/L,与中氟组和低氟组比较差异有统计学意义（P〈0.01）;高氟组的SOST阳性率为25.0%,与低氟组相比差异有统计学意义（P〈0.05）;损伤组、未损伤组和对照组的SOST分别为（4.870±0.504）μg/L、（5.100±0.627）μg/L和（5.234±0.603）μg/L,损伤组与对照组相比差异有统计学意义（P〈0.05）;高氟区对象血清SOST与ALP之间的线性相关系数为-0.319（P=0.001）;血清SOST与氟性骨损伤密切相关,SOST阳性者患氟性骨损伤的风险是SOST阴性者的2.417倍（P〈0.05）。结论]氟接触人群SOST明显降低,SOST与ALP活性及氟性骨损伤的发生发展有关,降低的SOST可能参与骨代谢及骨损伤过程,其确切机制有待进一步深入研究。

A Probable Correlation between Serum SOST Expression and Fluoride Bone Injury among Fluoride Exposed Population

Objective] To observe the expression of human serum sclerostin（SOST） in fluoride（F） exposed population,to study its correlation with alkaline phosphatase（ALP） and F bone injury,and try to explore the role of SOST in the process of F bone injury.Methods] General physical status,past and present health state of people exposed to fluoride were questionnaired.Their blood samples were collected from the cubital vein to measure the concentrations of SOST,ALP and blood fluoride（BF）.Their orthophoric forearm radiographies were taken.Statistical analysis was performed with SPSS 18.0.Results] The concentration of SOST in high BF group was（4.806±0.525） μg/L,which was significantly different from that in middle group and low group（P 0.01）.The SOST positive rate of high BF group was 25.0%,which was significantly different from that of low BF group（P 0.05）.The SOST concentration in injury group,non-injury group and control group were（4.870±0.504） μg/L,（5.100± 0.627） μg/L and（5.234±0.603） μg/L respectively,and there was significant difference between injury group and non-injury group（P 0.05）.The linear correlation coefficient of SOST and ALP in high fluoride area was r=-0.319（P=0.001）.Individuals with SOST positive rendered,significantly higher risk to develop F bone injury（OR=2.417） compared with those with SOST negative（P 0.05）.Conclusion] The serum SOST is significantly decreased in fluoride exposed population.SOST is related to ALP rising and fluorosis like bone injury.The reduced SOST may be involved in the development of fluorosis bone injury,however,its mechanism is still unrevealed.