肾细胞癌组织中CD146表达和微血管密度检测的临床意义

 目的　探讨肾细胞癌（RCC）组织中CD146 表达和微血管密度（MVD）与RCC临床病理参数的关系。方法　采用免疫组织化学方法分别检测43例RCC组织及20例正常肾组织中CD146蛋白表达情况和CD34标记的MVD，分析 CD146表达及MVD与RCC临床病理参数之间的相关性。结果　CD146蛋白在RCC组织中的阳性表达率为90.7 %（39／43），高于在正常肾脏组织中的阳性表达率30.0 %（6／20），两者差异有统计学意义（χ2＝27.77，P＜0.05）。其与肿瘤大小、转移明显相关（χ2＝7.57，r＝0.62、χ2＝19.99，均P＜0.05），而与肿瘤病理类型无关（χ2＝1.37，均P＞0.05）。光学显微镜200倍视野下，RCC组织的MVD为78.00±23.10 ,正常肾组织为23.05±7.93，两者差异有统计学意义（t＝13.93，P＜0.05）。其与肿瘤分期、转移相关（t＝2.37、2.10，均P＜0.05），而与肿瘤大小、病理类型无关（t＝1.33、1.46，均P＞0.05）。CD146蛋白在RCC组织中的表达与MVD在RCC组织中的水平有相关（r＝0.74 ，P＜0.05）。结论　CD146高表达与RCC血管形成有关；CD146表达及微血管生成与RCC的发生、发展及转移有关。

Clinical significance of CD146 protein expression and microvessel density detection in renal cell carcinoma

Objective To explore the relationship between CD146（MCAM）, microvessel density（MVD） in renal cell carcinoma（RCC） and its clinic-pathology, and to explore their correlation with clinic-pathologic parameter of RCC. Methods Immunohistochemisty was employed to determine the expression of CD146 and MVD in 43 RCC tissues and 20 normal control renal tissues. Results The positive expression of CD146 in RCC （90.7 %,39/43 ） was remarkably higher than that in normal renal tissue（30.0 %,6/20）（x2=27.77 , P〈0.05）. The expression of CD146 was not correlated with the category of RCC （x2=1.37,all P 〉0.05）,but had a significant correlation to（the tumor volume X2=7.57）clinical stage（r=0.62） and metastasis of RCC（x2=19.99,P 〈 0.05）. The MVD of RCC （78.00±23.10）/200HP]was significantly higher than that of normal renal tissue （23.05±7.93）/200HP].The MVD of CD146 was not correlated with the tumor volume and category of RCC （t= 1.33, t=1.46, au P 〉 0.05）, but had a significant eorrelation to clinical stage and metastasis of RCC （t=2.37, t=-2.10, P 〈 0.05）. There was a positive correlation between expression of C D146 and MVD in RCC（r=-0.74 , P 〈 0.05）. Conclusion The overexpression of CD146 in RCC has a significant relation with tumor angiogenesis. The expression of CD146 and angiogenesis might serve as an important indicator of the development, progress and metastasis of RCC.