强致病岛在多重耐药革兰阴性杆菌中的分布及序列分析

目的探讨强致病岛（HPI）fyuA—irp2基因簇在多重耐药革兰阴性杆菌中的分布及分子生物学特征。方法用多重聚合酶链式反应（PCR）技术扩增84株菌的fyuA—irp2基因簇，对产物进行DNA测序。结果irp1、irp2、irp3、irp4及，fyuA的总检出率分别是：40．48％、41．67％、5．95％、0％及16．67％；ECO6748、Kp7151及PAE7之fyuA蛋白质序列与YP_853080的相比有100％同源；克雷伯菌蜘49及Kp之irp2与AAA27636．1的同源性高（99％），而大肠埃希菌ECO4、ECO7与1176840的相符率低（90％），GenBank登陆号分别为：FJ211852及FJ211851；Kp51、Kp10及和49之irp1均与AL590842有99％同源；EC03、Kp51、Kp10及脚49之irp3与CAA73128相比有97％的同源。同种菌株突变的位点大致相同，不同种菌株间的突变差异较大。结论在多重耐药革兰阴性杆菌中检出HPI，fyuA-irp2基因簇有不同程度的突变和缺失。

The distribution of high pathogenicity island in multiple-drug-resiatance gram-negative bacilli and analyzation of DNA sequence

Objective To investigate the distribution of high pathogenicity island(HPI)in multiple-drug-resistance gram-negative bacilli and analyze the protein sequence.Methods To amplify thefyuA-irp2 gene cluster of the 84 isolates by multiple polymerase chain reaction(PCR),the product was subsequently sequenced.Results The positive rate ofirpl,irp2,irp3,irp4 and fyuA was 40.48％,41,67％,5.95％,O％and 16.67％,respectively.Theamino sequence offyuA comefromEC06748,Kp7151 and PAE7 was usedto compare with AL590842,there are 100％identities.Amino sequence ofirp2 come from Kp49 and Kp51 have 99％identities with AAA27636.1,but amino sequence of irp2 come from EC04 and EC07 only have 90％identities with 1176840.The GenBank accession number is FJ211852 and FJ211851.Amino sequence ofirpl come fromKp 10,Kp49 and Kp51 have 99％identities with AL590842。and amino sequence ofirp3 come from EC03,Kp51,Kp10 and Kp49 have 97％identities with CAA73128.There are the same mutation among the same species,and different mutation among different species.Conclusion There was different extant mutant lost in thefy～t-i,v2 gene cluster in multiple-drug-resistanee gram-negative bacilli.