Measurement of serum IgG4 levels by a competitive immunoenzymatic assay with monoclonal antibodies |
| |
| Authors: | Pierre Aucouturier,Fran?oise Danon,Maryvonne Daveau,Bernard Guillou,Alfred Sabbah,Jacques Besson,Jean Louis Preud&#x homme |
| |
| Affiliation: | 1. Laboratoire d''Immunologie et Immunopathologie, C.H.R.U. La Milétrie, Poitiers, France;2. Laboratoire d''Immunochimie et Immunopathologie (INSERM U 108), Hôspital Saint-Louis, Paris, France;3. Unité de Recherches sur la Génetique des Protéines Humaines (INSERM U 78), Bois-Guillaume, France;4. Laboratoire de Thermique de l''ENSMA (LA 191 du CNRS), Poitiers, France;5. Laboratoire d''Immunoallergologie, C.H.U.R. d''Angers, France;6. Département de Statistique, Faculté de Médecine, Poitiers, France |
| |
| Abstract: | A competitive indirect ELISA is described for the measurement of IgG4 levels. It uses a monoclonal anti-subclass antibody and purified monoclonal IgG4 as standards. This method is sensitive and reproducible and more accurate than hemagglutination inhibition and radial immunodiffusion. Serum IgG4 levels in 173 normal adults were < 0.01–2.1 mg/ml (mean 0.30 mg/ml) in women and < 0.01–1.87 mg/ml (mean 0.465 mg/ml) in men. |
| |
| Keywords: | immunoglobulin subclasses competitive ELISA monoclonal antibodies |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
