| CTLA4—Ig影响T细胞功能及机理的初步研究 |
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| 引用本文： | 郑宇,黎燕,沈倍奋,苏琴,孙英勋,张纪岩.CTLA4—Ig影响T细胞功能及机理的初步研究[J].中国免疫学杂志,2001,17(9):455-458,462. |
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| 作者姓名： | 郑宇 黎燕 沈倍奋 苏琴 孙英勋 张纪岩 |
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| 作者单位： | 1. 解放军302医院免疫室,北京,100039
2. 军事医学科学院基础所免疫室,北京,100850 |
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| 基金项目： | 国家"863"计划生物领域资助课题(102-09-01-03) |
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| 摘 要： | 目的：研究CTLA4-Ig融合蛋白对外周血T淋巴细胞活化的抑制作用及机理。方法：分离新鲜人外周血单个核细胞,观察CTLA4-Ig对淋巴细胞转化和混合淋巴细胞培养的作用,用流式细胞术观察CTLA4-Ig对T细胞表面CD25表达的影响,最近通过EMSA方法检测激活T细胞中核蛋白的变化。结果：CTLA4-Ig能够抑制淋巴细胞转化和混合淋巴细胞反应,减低T细胞表面CD25的表达,抑制核内与IL-2表达相关的RE/AP结合因子的活化。结论：CTLA4-Ig通过多种信号途径对T细胞的活化起作用,其机制之一是抑制核内结合于IL-2增强子RE/AP位点的核因子的活化。
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| 关 键 词： | CTLA4－Ig T细胞活化 核蛋白RE／AP |
| 文章编号： | 1000-484X(2001)09-0455-05 |
The preliminary study on the effect of CTLA4-Ig on T cell function and its mechanism |
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| ZHENG Yu,LI Yan,SHEN Bei Fen et al.The preliminary study on the effect of CTLA4-Ig on T cell function and its mechanism[J].Chinese Journal of Immunology,2001,17(9):455-458,462. |
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| Authors: | ZHENG Yu LI Yan SHEN Bei Fen |
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| Institution: | ZHENG Yu,LI Yan,SHEN Bei Fen et al.Department of Immunology,302 Hospital of PLA,Beijing 100039 |
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| Abstract: | Objective:To study the inhibited function of CTLA4 Ig on T cell activation and its mechanism Methods:After separating fresh PBMC,the effects of CTLA4 Ig on T lymphocyte transformation and mixed lymphocyte reaction were analyzed,and its inhibitory effect on the CD25 expression on T cell surface was observed At last,the changes of the nuclear protein in active T cell were analyzed by the EMSA method.Results:CTLA4 Ig has an inhibitory effect on the lymphocyte transformation,MLR and CD25 expression on T cell surface,and it can inhibite the activation of RE/AP binding factor related to IL 2 expression. Conclusion:CTLA4 Ig inhibit the activation of T cell by multipathway,one of the mechanism might be its inhibitory effect on the activation of RE/AP binding factor. |
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| Keywords: | CTLA4 Ig Activation of T cell RE/AP |
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