| 葡萄糖和胰岛素对脂肪细胞内脂素表达的作用 |
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| 引用本文： | 王坤,王文汇,陈淼,陈福莲,娄宁.葡萄糖和胰岛素对脂肪细胞内脂素表达的作用[J].山东大学学报(医学版),2009,47(5):75-77. |
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| 作者姓名： | 王坤 王文汇 陈淼 陈福莲 娄宁 |
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| 作者单位： | 王坤,陈淼,陈福莲,WANG Kun,CHEN Miao,CHEN Fu-lian(山东大学医学院,济南,250012);王文汇,娄宁,WANG Wen-hui,LOU Ning(山东大学附属济南市中心医院内分泌科,济南,250013)
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| 摘 要： | 目的 观察脂肪细胞经不同浓度葡萄糖和胰岛素干预后内脂素表达的改变，探讨内脂素在糖尿病发病过程中的作用。 方法 培养3T3-L1前脂肪细胞，并诱导分化成成熟脂肪细胞。采用不同浓度葡萄糖、胰岛素孵育脂肪细胞48h，RT-PCR和western blot技术检测3T3-L1脂肪细胞中内脂素mRNA和蛋白的表达水平 结果 与对照组比较，随着葡萄糖浓度的增加，3T3-L1脂肪细胞内脂素mRNA和蛋白的表达量下降（P均＜0.05）；在不同浓度胰岛素作用下，3T3-L1脂肪细胞内脂素mRNA和蛋白的表达量改变无统计学意义（P＞0.05）。 结论 高糖状态可抑制体外培养的脂肪细胞内脂素的表达，胰岛素浓度的改变对内脂素表达无影响。
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| 关 键 词： | 葡萄糖 胰岛素 脂细胞 内脂素 |
| 收稿时间： | 2008-11-20 |
Effect of glucose and insulin on visfatin expression in 3T3-L1 adipocytes |
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| WANG Kun,WANG Wen-Hui,CHEN Miao,CHEN Fu-Lian,LOU Ning.Effect of glucose and insulin on visfatin expression in 3T3-L1 adipocytes[J].Journal of Shandong University:Health Sciences,2009,47(5):75-77. |
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| Authors: | WANG Kun WANG Wen-Hui CHEN Miao CHEN Fu-Lian LOU Ning |
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| Institution: | 1. School of Medicine， Shandong University, Jinan 250012， China；2. Department of Endocrinology， Jinan Central Hospital Affiliated to Shandong University， Jinan 250013， China |
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| Abstract: | Objective To investigate the effects of different concentrations of glucose and insulin on expressions of visfatin mRNA and protein in 3T3-L1 adipocytes and to explore the role of visfatin on diabetes mellitus.Methods 3T3-L1 preadipocytes were cultured in vitro and differentiated.Mature adipocytes were incubated with different concentrations of glucose and insulin for 48 hours.Then total cellular RNA and protein were extracted,and expressions of visfatin mRNA and protein were determined by semi-quantitative... |
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| Keywords: | Glucose Insulin 3T3-L1 Cells Visfatin |
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