人淋巴细胞转输SCID小鼠抗人脑胶质瘤特性分析

目的 探讨人外周血淋巴细胞转输给T和B淋巴细胞均先天性缺乏的SCID鼠后抗胶质瘤的免疫学特征。方法 采用APAAP、间接荧光原位杂交和流式细胞计数等检测转输在SCID鼠体内的人淋巴细胞分布、亚群变化和抗胶质瘤细胞毒活性。结果 尾静脉转输后CD3^ 、CD8^ 和HLA－Dr^ 等具有细胞毒活性细胞大部分分布在外周血和脾脏中，而腹腔转输者大部分在腹腔，这些细胞的功能维持1周时达高峰，在第2周下降时重复转输又可得到加强并具攻击肿瘤活性。结论 淋巴细胞免疫人化SCID模型是目前研究人胶质瘤免疫治疗的理想工具。

The analysis ofanti-glioma characteristics of human peripheral blood lymphocytes

Objective　To probe into the anti-glioma immunity characteristics of SCIB mice which are lack of T　＆ B lymphocytes after transferring HuPBLs．Methods　The distribution，subclass variety and cytotoxic activities of human lymphocytes transferred in SCID mice were detected by the methods of APAAP，indirect fluorescence in-situ hybridization and cytotoxicity analysis．Results　The cytotoxic human lymphocytes such as CD3＋，CD8＋ and HLA-Dr＋ transferred by i．v． distributed mostly in peripheral blood and spleen of SCID mice，while lymphocytes transferred by i．p． distributed mostly in abdominal cavity．The anti-tumor immunity of these cells can stay for one week to reach the summit and decline in the second week，which can be strengthened by transferring repeatedly．Conclusion　This HuPBL-SCID mice inlaid model is an ideal model to study biotherapy of human glioma now．