HBV感染者血清中IL-4、IL-18水平的测定及意义

目的探讨白细胞介素-4(interleukin-4,IL-4)、白细胞介素-18(interleukin-18,IL-18)在HBV感染中的作用。方法选择2003年1月至2004年3月在珠海市人民医院住院的乙型肝炎患者52例,男性32例,女性20例,年龄18～58岁,平均年龄35岁。对照组20例为健康体检者。病毒性肝炎诊断标准按2000年西安第十次全国病毒性肝炎与肝病学术会议讨论修订的《病毒性肝炎防治方案》进行分型诊断。用ELISA法测定52例乙型肝炎患者和20例健康者血清中IL-4、IL-18水平,荧光定量PCR法测定肝炎患者血清中HBV-DNA的含量。结果重型肝炎组、慢性肝炎组血清中IL-4、IL-18水平明显高于健康对照组(P<0.05);但血清IL-4水平两组之间比较无统计学意义(P>0.05);IL-18水平两组间比较有显著性差异(P<0.01)。重型肝炎组IL-4/IL-18比慢性肝炎组降低更明显(P<0.05)。高病毒组与低病毒组比较血清IL-18水平有显著性差异(P<0.05),而血清IL-4水平无统计学意义(P>0.05)。结论IL-4、IL-18可能参与慢性乙型肝炎的免疫应答,在清除感染的HBV的同时也造成肝细胞的损伤。

The relationship between serum IL-4 and IL-18 level in patients with hepatitis B virus infection

Objective To evaluate the possible roles of IL-4 and IL-18 in hepatitis B virus (HBV) infection. Methods Fifty-two patients of hepatitis B virus were selected from January 2003 to March 2004 in the people's hospital of Zhuhai, of which were 32 males, 20 females, aging from 18 to 58 years old, average 35 years old. The control group was 20 healthy people. Diagnostic standard of hepatitis virus accorded to the revised in the prevention project of hepatitis virus in 2000. The level IL-4 and IL-18 in serum was measured using ELISA, and HBV-DNA content in serum was detected by FQ-PCR. Results There was significantly higher level in serum IL-4 and IL-18 between the fulminant hepatitis patients and chronic hepatitis patients and normal control (P< 0.05). There was significance in serum IL-18 level between the fulminant hepatitis patients and chronic hepatitis patients (P< 0.01). Serum IL-4?IL-18 level was the highest in fulminant hepatitis patients, and the lowest in normal individuals. In addition, the serum IL-18 level in patients with low HBV-DNA content was higher than that in patients with high HBV-DNA content. Conclusion IL-4 and IL-18 may share the host immunity against HBV, and is associated with HBV clearance and liver damage .