六味地黄丸血中移行成分对氢化可的松致大鼠肾虚动物模型的保护作用

目的:通过研究六味地黄丸血中移行成分对氢化可的松致大鼠肾虚动物模型的保护作用,阐明六味地黄丸补肾的药效物质基础。方法:70只大鼠随机分为空白组、模型组、总组分组、混合组分组、5-羟甲基-2-糠酸(5-HMFA)组、丹皮酚组和六味组共7个实验组,按5 mL/kg体重连续9 d分别灌胃给予蒸馏水、蒸馏水、总组分药液、混合组分药液、5-HMFA药液和丹皮酚药液;第5~9天,除空白组皮下注射生理盐水外,其他各组按5 mL/kg体重每天皮下注射氢化可的松注射液25 mg/kg,于实验的第10天测定体重等10项评价指标。结果:与模型组相比,各给药组动物的检测指标均有明显改善,其中莫诺苷、獐牙菜苷、马钱子苷的混合物组作用最显著。结论:初步确定所分离得到的六味地黄丸的血中移行成分是六味地黄丸补肾的药效物质基础,其中以莫诺苷,獐牙菜苷和马钱子苷的作用最为明显,是补肾的核心成分。

Protective Effect of Liuwei Dihuang Wan and its Constituents Absorbed into Blood After Oral Administration on Hydrocortisone Inducing Renal Deficiency in Rat

Objective:To elucidate bioactive constituents for invigorating the kidney in Liuwei Dihuang Wan based on study on protective effect of Liuwei Dihuang Wan and its constituents absorbed into blood after oral administration on hydrocortisone inducing renal deficiency in rat.Method: Seventy rats were randomly divided into seven groups including control,model,total components,5-HMFA,mixture containing morroniside,sweroside and loganin,paeonol and LW group.For continous 9 days,every group rat was respectively orally administrated with distilled water,distilled water,total components,5-HMFA mixture containing morroniside,sweroside and loganin,paeonol and LW,from 5 day to 9 day,control group rat was subcutaneously injected with sodium chloride at a dose of 5 ml/kg body weight,the other groups rat was subcutaneously injected with hydrocortisone at a dose of 25 mg/kg body weight(5 ml/kg).On 10~(th) day,collected samples for analyzing 10 batches of indexes.Result: Every group drugs all improved such 10 batches of indexs in a large,the effect of mixture containing morroniside,sweroside and loganin group is most significantly.Conclusion: It is found that determined constituents absorbed into blood after oral administration of Liuwei Dihuang Wan is mainly bioactive constituents for invigorating the kidney,and the bioactivities of morroniside,sweroside and loganin is most perferred.