| 小鼠脊髓脱髓鞘病变后LINGO-1的动态表达变化 |
| |
| 引用本文： | 张永杰,顾振,古慕峰,王鹤鸣,韩群颖,唐敏峰.小鼠脊髓脱髓鞘病变后LINGO-1的动态表达变化[J].解剖学研究,2009,31(4):249-252,258. |
| |
| 作者姓名： | 张永杰 顾振 古慕峰 王鹤鸣 韩群颖 唐敏峰 |
| |
| 作者单位： | 南京医科大学人体解剖学系,江苏,南京,210029 |
| |
| 摘 要： | 目的观察LINGO-1在溶血卵磷脂注射诱导脊髓脱髓鞘病变小鼠脊髓中的动态表达变化。方法模型组小鼠接受1%溶血卵磷脂注射至T8-9和T9-10胸椎节段的脊髓双侧腹外侧区,诱导脱髓鞘病变。甲苯胺蓝染色观察病变范围;实时定量RT-PCR和western blot检测病变区脊髓LINGO-1的表达变化。结果溶血卵磷脂注射1d后,病变区脊髓LINGO-1mRNA和蛋白表达上调均最为显著,其后逐渐下降,LINGO-1mRNA在2周后接近空白对照,而LINGO-1蛋白至4周时仍略高于空白对照。结论LINGO-1在脊髓脱髓鞘病变早期即上调并呈一定规律性改变,提示其与脊髓脱髓鞘病变有关;对于治疗脊髓脱髓鞘病变,LINGO-1拮抗剂应在损伤早期使用。
|
| 关 键 词： | 脊髓损伤 脱髓鞘病变 LINGO-1 溶血卵磷脂 |
Dynamic expression of LINGO-1 after spinal cord demyelination in mice |
| |
| ZHANG Yong-jie,GU Zhen,GU Mu-feng,WANG He-ming,HAN Qun-ying,TANG Min-feng.Dynamic expression of LINGO-1 after spinal cord demyelination in mice[J].Anatomy Research,2009,31(4):249-252,258. |
| |
| Authors: | ZHANG Yong-jie GU Zhen GU Mu-feng WANG He-ming HAN Qun-ying TANG Min-feng |
| |
| Institution: | ZHANG Yong-jie,GU Zhen,GU Mu-feng,WANG He-ming,HAN Qun-ying,TANG Min-feng. Department of Human Anatomy,Nanjing Medical University,Nanjing 210029 China |
| |
| Abstract: | Objective To investigate the dynamic expression of LINGO-1 after spinal cord demyelination induced by lysolecithin injection in mice. Methods 39 female C57Bl / 6 mice were used in this study and were randomly divided into 4 groups: normal controls (group A: 6 mice), sham operated controls (group B: 6 mice), operated groups (group C: 24 mice), and model detected group (group D: 3 mice). Animals in group C and D were treated with lysolecithin injection. Then the mice in group C were divided into 1day, 1week, ... |
| |
| Keywords: | LINGO-1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
