MicroScan AutoScan-4系统筛选超广谱β-内酰胺酶的可靠性评价

目的 对MicroScan AutoScan-4（MS-Auto4）系统筛选大肠埃希菌（Eco）和肺炎克雷伯菌（KDn）产超广谱β-内酰胺酶（ESBLs）的可靠性评价。方法 在MS-Auto4系统对临床标本分离的150株Eco和65株Kpn进行ESBLs筛选的同时，用纸片扩散法做表型确证试验。结果 150株Eco和65株Kpn中，经确证ESBLs阳性的有40株和21株，系统提示为可疑产为ESBLs的有60株和26株，可筛选出全部ESBLs阳性菌株，灵敏度为100％，特异度为83．8％，假阳性率为16．2％，没有造成漏筛。结论 用MS-Auto4系统对Eco和Kpn进行鉴定及药敏试验的同时，可利用其专家系统对ESBLs进行筛选，有助于临床及早翻订合理的治疗方案。

Evaluation of Microscan Autoscan-4 system on screening of extended-spectrum beta-lactamases

Objective To evaluate the reliability of Microscan Autoscan-4 system (MS-Auto4) on screening of extended-spectrum beta-lactamases(ESBLs) in common bacteria. Methods Confirmatory test for EBSLs in 150 Escherichia coli(Eco) and 65 Klebsiella pneumoniaes(Kpn) was detected by disk diffusion(K-B) method when screening test was performed by MS-Auto4. Results ESBLs-producing strains confirmed by K-B method were 40 and 21 respectively in 150 Eco and 65 Kpn, suspected ESBLs-producing strains screened by MS-Auto4 were 60 and 26. MS-Auto4 could screen all the ESBLs-producing strains, the sensitivity and specificity were 100% and 83.8%, and the false positive rate was 16.2%, no strains were false to be screened. Conclusions MS-Auto4 can screen for ESBLs when it detect on common bacteria.