去甲基雏菊叶龙胆酮对Ox-LDL诱导内皮细胞衰老的影响

目的研究[口山]酮化合物去甲基雏菊叶龙胆酮（DMB）对氧化型低密度脂蛋白（Ox-LDL）致内皮细胞衰老的影响。方法培养人脐静脉内皮细胞（HUVECs），Ox-LDL（100μg·mL^-1）处理48h后，检测β-半乳糖苷酶活性（染色法）和端粒酶活性（TRAP-银染法）评价细胞衰老状态；使用荧光染料H2DCF检测细胞内氧自由基生成。结果Ox-LDL（100μg·mL^-1）处理48h后，β-半乳糖苷酶染色阳性细胞数显著增加，而端粒酶活性显著降低。预先1h给予不同浓度的DMB（1、3和10μmol·L^-1）并孵育48h可显著抑制Ox-LDL诱导的内皮细胞β-半乳糖苷酶染色阳性细胞数增加和端粒酶活性降低。Ox-LDL（100μg·mL^-1）孵育HUVECs 48h，可明显增强细胞内活性氧族（ROS）水平，其效果能被预处理DMB所逆转。结论DMB抑制Ox-LDL诱导的内皮细胞衰老，其作用可能与抑制氧化应激有关。

Demethylbellidifolin inhibits oxidative low-density lipoprotein induced senescence of endothelial cells

Objective To investigate the effect of demethylbellidifolin （DMB）, a xanthone compound, on oxidative low-density lipoprotein （Ox-LDL） -induced senescence of endothelial cells, Methods Human umbilical vein endotheli al cells （HUVECs） were cultured and treated with Ox-LDL （100μg · mL^-1） for 48 h to induce senescence reflected by both senescence-associated beta-galactosidase activity and telomerase activity. The level of intracellular reactive oxygen species （ROS） was determined by H2DCF, an oxidant-sensitive fluorscent indicator. Results Treatment with Ox-LDL （100μg · mL^-1） for 48 h significantly increased heta-galactosidase-positive cell ratio and reduced telomerase activity, which could be markedly attenuated by pretreatment with DMB （1, 3 and 10μmol · L^-1 ）. Also, the elevation of intracellular ROS production elicited by Ox-LDL was significantly suppressed by pretreatment with DMB. Conclusion DMB can inhibit Ox-LDI=induced senescence of endothelial cells, and such effect of DMB may be related to inhibiting intracellular ROS production.