HPLC法测定牛蒡子提取物中咖啡酰奎宁酸类化合物的含量

目的：以高效液相色谱法测定牛蒡子提取物中咖啡酰奎宁酸类化合物的含量。方法：色谱柱为Welch welchromC18（250mm×4.6mm，5μm），流动相为乙腈：0.1%磷酸（乙腈16%～18%0～60min梯度洗脱乙腈20%65min等度洗脱），流速为1.0ml·min-1，检测波长为327nm，柱温为室温。结果：3，5-二咖啡酰奎宁酸、1，5-二咖啡酰奎宁酸、3，4-二咖啡酰奎宁酸的进样量分别在4.28～12.84μg（r=0.9992）、4.16～12.48μg（r=0.9991）、4.20～12.60μg（r=0.9992）范围内与各自峰面积积分值呈良好线性关系；平均回收率分别为97.70%、98.50%、98.62%，RSD为1.80%、1.90%、1.20%。结论：本方法简便、快速、准确，可用于牛蒡子提取物中咖啡酰奎宁酸类化合物的质量控制。

Content Determination of Caffeoylquinic Acids Compounds from the Extractive of Fructus Arctii by HPLC

Objective：To determine the contents of Caffeoylquinic Acids Compounds from the extractive of Fructus Arctii by HPLC. Methods：The determination was performed on Welch welchromC18 （250mm ×4.6mm,5μm）column with mobile phase consisted of acetonitrile：0.1% phosphoric acid acetonitrile16%~18% 0~60 min gradient elution, acetonitrile20% 65 min isocratic elution],at flow rate of 1.0ml·min-1.The detection wavelength was set at 327 nm and the column temperature was room temperature. Results：The linear range of 3,5-dicaffeoylquinic acid was 4.28~12.84μg （r=0.9992）with an average recovery of 97.70%（RSD=1.80%,n=6）. The linear range of 1,5-dicaffeoylquinic acid was 4.16~12.48μg （r=0.9991）with an average recovery of 98.50%（RSD=1.90%,n=6）. The linear range of 3,4-dicaffeoylquinic acid was 4.20~12.60μg（r=0.9992）with an average recovery of 98.62%（RSD=1.20%,n=6）.Conclusion：The method is simple, rapid and accurate for the quality control of Caffeoylquinic Acids Compounds in the extractive of Fructus Arctii.