哌甲酯控释剂对自发性高血压大鼠纹状体胶质细胞源性神经营养因子mRNA表达的作用

目的:哌甲酯控释剂对自发性高血压大鼠纹状体胶质细胞源性神经营养因子((GDNF)mRNA基因表达的影响。方法:将SHR随机分为模型对照组(MC组)和哌甲酯控释剂组(MPH组)。各组按设计剂量给药14 d后,取纹状体组织和血清,采用高效液相法检测多巴胺(DA)的含量、原位杂交法检测纹状体GDNF mRNA的基因表达。结果:哌甲酯控释剂组的纹状体神经元的细胞膜及细胞浆GDNF mRNA基因表达明显增强,模型对照组未见明显表达;哌甲酯控释剂组与模型对照组比较,血清和纹状体中多巴胺的含量显著升高。结论:哌甲酯控释剂有促进纹状体GDNF mRNA的基因表达和增加纹状体和血清中多巴胺的含量的作用,这可能是其治疗ADHD的作用机制之一。

Effects of methylphenidate hydrochloride controlled-release tablets on the expression of GDNF mRNA in striate body of the spontaneously hypertensine

Objective To investigate the effects of methylphenidate hydrochloride controlled-release tablets on the expression of GDNF mRNA in striate body of the Spontaneously hypertensine rats.Methods SHR rats were randomized into MC group and MPH group,and received gastric gavage of corresponding drugs according to the experimental design for 14 days.HPLC was used to detecte the content of DA in striate body and sera,and GDNF mRNA in striate body was detected by in situ hybridization after treatment.Results GDNF mRNA was positively expressed in striate body of rats in MPH group,and was not expressed in those in MC group.The content of DA in striate body and sera of rats in MPH group was significantly higher than that in MC group.Conclusion MPH can increase the expression of GDNF mRNA and the content of DA in striate body and sera in SHR rats,which may be involved in the mechanisms of its treating effect on ADHD.