重组人肿瘤坏死因子α衍生物治疗鼠脑胶质瘤

 目的　探讨重组人肿瘤坏死因子α衍生物 (rhTNF α)治疗鼠脑胶质瘤的有效性。方法　通过改造人肿瘤坏死因子α形成rhTNF α ,体外培养鼠脑胶质瘤细胞 ,加入不同浓度rhTNF α,MTT法测定细胞存活率 ,颅内立体定向种植C6细胞制成鼠脑胶质瘤模型 ,10天后MRI增强测量肿瘤大小 ,腹腔注射rhTNF α ,治疗后第 8天、15天、2 1天分别用MRI增强测量肿瘤大小。结果　体外实验 :加入rhTNF α 5天后 ,大部分C6细胞死亡 ,细胞存活率随rhTNF α的递增而降低。体内实验 ,治疗 8天后肿瘤即开始缩小 ,治疗 2 1天后 ,MRI未见明显肿瘤强化灶。结论　rhTNF α能有效治疗鼠脑胶质瘤。

Derivative of recombinant human tumor necrosis factor-α therapy for rat glioma

Objective To investigate the effects of derivative of recombinant human tumor necrosis factor-α(rhTNF-α) therapy for rat glioma. Methods rhTNF-α were created by modifying TNF-α, were cultured in culture medium conclude rhTNF-α in different concertration. The livability of cells was determined by MTT technique five days later and implanted C 6 cells in rat s brain to establish glioma model. rhTNF-α were intraperitoneally injected 10 days after implanted. The volume of glioma were measured by MRI 10 days a...