P~(16)蛋白在宫颈癌中的表达及其临床意义

目的：研究抑癌基因P16与宫颈癌发生、发展及患者预后的关系。方法：用免疫组化ABC法检测了10份正常宫颈组织、16份慢性宫颈炎及123份宫颈鳞癌组织中P16的表达情况。结果：P16在宫颈癌中检出率为43．90％，明显低于正常宫颈（80．00％）及宫颈炎组织（68．75％），P＜0．01。其中伴淋巴结转移的宫颈癌中P16蛋白表达率（22．22％）是显著低于无淋巴结转移者（61．11％），P＜0．05。P16蛋白表达阳性者5a存活率为52．63％，而阴性者仅为29．79％，P＜0．05。结论：P16蛋白的突变与缺失可能与宫颈癌的发生、发展密切相关。

Expression of p~16 protein in cervical carcinoma and its clinical significance

To research the relationship among tumor suppressor gene p16 expression and cervical carcinoma occurrence, development and patient's prognosis. Methods: ABC immunohistochemistry was employed to detect the expression of p16 protien in tissues of 10 normal uterine cervix, 16 chronic cetvicitis and 123 cervical squamous carcinoma. Results:The positive rate of p16 was significantly lower in carcinoma (43. 90% ) than that in normal uterine cervix (80. 00 % ) , cervicitis(68. 75 % ), P<0. 01, the positive rate in cervical carcinoma with lymph node metastasis(22. 22 % )was markedly lower than that in nonmetastasis(61. 11 % ), P<0. 05. Patients with positive p16 expression had a much high 5 a survival rate (52. 63% ) than that with negative expression (29. 79% ), P < 0. 05.Conclusion: The mutation and deletion of p16 protein may be related to the genesis and development of the uterine cervical carcinoma.