| 鱼藤酮对星形胶质细胞缝隙连接耦联功能的影响 |
| |
| 引用本文： | 刘辉,尹芳秋,郭魁亮,许崇亮,贾庆军.鱼藤酮对星形胶质细胞缝隙连接耦联功能的影响[J].中国工业医学杂志,2009,22(3):168-170+241. |
| |
| 作者姓名： | 刘辉 尹芳秋 郭魁亮 许崇亮 贾庆军 |
| |
| 作者单位： | 中国人民解放军白求恩军医学院卫勤教研室; |
| |
| 基金项目： | 白求恩军医学院院级课题(200706) |
| |
| 摘 要： | 目的观察鱼藤酮对星形胶质细胞缝隙连接耦联功能的影响。方法建立星形胶质细胞与常用的神经细胞株PC12细胞共培养鱼藤酮染毒模型,应用划痕染料标记示踪技术观察染毒星形胶质细胞缝隙连接耦联功能的变化,采用RT-PCR法检测CX43 mRNA的表达,Western Blot技术观察CX43蛋白活性变化。结果随着染毒剂量的增加,鱼藤酮对星形胶质细胞缝隙连接耦联功能的抑制作用愈发明显;与正常对照组比较,0.5、1.0μmol/L鱼藤酮染毒组CX43 mRNA和蛋白表达均显著降低(P<0.01)。结论鱼藤酮可引起星形胶质细胞CX43表达水平降低,显著抑制细胞缝隙连接耦联功能,这可能是其诱导神经细胞损伤的原因之一。
|
| 关 键 词： | 鱼藤酮 星形胶质细胞 缝隙连接功能 划痕染料标记示踪技术 |
Effect of rotenone on intercellular gap junction function of astrocytes |
| |
| LIU Hui,YIN Fang-qiu,GUO Kui-liang,XU Chong-liang,JIA Qing-jun.Effect of rotenone on intercellular gap junction function of astrocytes[J].Chinese Journal of Industrial Medicine,2009,22(3):168-170+241. |
| |
| Authors: | LIU Hui YIN Fang-qiu GUO Kui-liang XU Chong-liang JIA Qing-jun |
| |
| Institution: | LIU Hui,YIN Fang-qiu,GUO Kui-liang,XU Chong-liang,JIA Qing-jun(Department of Health Service,Norman Bethune Military Medical College,Shijiazhuang 050081,China) |
| |
| Abstract: | Objective To study the effects of rotenone on intercellular gap junction function of astrocytes.Methods Astrocytes isolated from newborn rats were cocultured with rotenone and a common cell strain-PC 12 cells;the intercellular gap junction function of rotenone-exposed astrocytes was observed by scrape-loading and dye transfer technique(SLDT),and the expression of CX43 mRNA and protein were detected by RT-PCR and Western Blot.Results The results showed that the the intercellular gap junction function of astr... |
| |
| Keywords: | Rotenone Astrocyte Gap junction function Scrape-loading and dye transfer technique(SLDT) |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国工业医学杂志》浏览原始摘要信息 |
| 点击此处可从《中国工业医学杂志》下载免费的PDF全文 |
|
