| 远志中西伯利亚远志糖A5和西伯利亚远志糖A6的含量测定方法研究 |
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| 引用本文： | 孙长清,彭晓敏,朱乃亮,姜艳艳,康丽丽,崔晓辉,郭小红,石任兵. 远志中西伯利亚远志糖A5和西伯利亚远志糖A6的含量测定方法研究[J]. 中国中药杂志, 2012, 37(11): 1607-1609 |
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| 作者姓名： | 孙长清 彭晓敏 朱乃亮 姜艳艳 康丽丽 崔晓辉 郭小红 石任兵 |
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| 作者单位： | 北京中医药大学中药学院,北京,100102 |
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| 基金项目： | 国家自然科学基金项目(81102776);国家"重大新药创制"科技重大专项(2009ZX09502-006);国家"十二五"科技支撑计划项目(2012BAI29B06);北京中医药大学新团队项目(2011-CXTD-12);北京中医药大学研究基地建设项目(2011-JDJS-13) |
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| 摘 要： | 目的:建立高效液相色谱法同时测定远志中西伯利亚远志糖A5和西伯利亚远志糖A6含量的方法。方法:艾杰尔Promosil C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%磷酸溶液(10∶90)等度洗脱,检测波长330 nm,流速1mL.min-1,柱温30℃。结果:西伯利亚远志糖A5和西伯利亚远志糖A6分别在0.008 7～0.069 4 g.L-1(r=0.999 3),0.009 0～0.072 3 g.L-1(r=0.999 1)时线性关系良好,平均加样回收率依次为101.7%,97.8%,RSD为1.7%,1.6%。结论:该方法操作简便、快速、准确、可靠,适用于远志中西伯利亚远志糖A5和西伯利亚远志糖A6的含量测定和远志药材的质量控制。
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| 关 键 词： | 远志 西伯利亚远志糖A5 西伯利亚远志糖A6 HPLC 含量测定 |
| 收稿时间： | 2011-10-17 |
Quantitative determination of sibricose A5 and sibricose A6 in Polygalae Radix |
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| SUN Changqing,PENG Xiaomin,ZHU Nailiang,JIANG Yanyan,KANG Lili,CUI Xiaohui,GUO Xiaohong and SHI Renbing. Quantitative determination of sibricose A5 and sibricose A6 in Polygalae Radix[J]. China Journal of Chinese Materia Medica, 2012, 37(11): 1607-1609 |
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| Authors: | SUN Changqing PENG Xiaomin ZHU Nailiang JIANG Yanyan KANG Lili CUI Xiaohui GUO Xiaohong SHI Renbing |
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| Affiliation: | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China |
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| Abstract: | Objective: To establish the method for quantitative determination of sibricose A5 and sibricose A6 in Polygalae Radix by HPLC.Method: The ultrasonic extracting method was applied in sample pre-treatment.The HPLC procedure was performed on the chromatographic column of Agela Promosil C18(4.6 mm×250 mm,5 μm),the mobile phase was acetonitrile-0.1% phosphoric acid water solution(10∶90).The detection wavelength was 330 nm and flow velocity was 1 mL·min-1.The column temperature was 30 ℃.Result:The method has good linearity in the ranges of 0.008 7-0.069 4 g·L-1(r=0.999 3)for sibricose A5,0.009 0-0.072 3 g·L-1(r=0.999 1)for sibricose A6.The average recoveries of sibricose A5 and sibricose A6 were 101.7%,97.87%,with the RSD of 1.7%,1.6%,respectively.Conclusion: The method was simple,quick accurate and reliable.It is appropriate for the quantitative determination of sibricose A5 and sibricose A6 in Polygalae Radix. |
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| Keywords: | Polygalae Radix sibricose A5 sibricose A6 HPLC quantitative determination |
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