| ISSR标记研究云南产牛奶菜属几种药用植物的亲缘关系 |
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| 引用本文： | 管志斌,张丽霞,宋美芳,李海涛,张忠廉. ISSR标记研究云南产牛奶菜属几种药用植物的亲缘关系[J]. 中国中药杂志, 2012, 37(11): 1550-1552 |
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| 作者姓名： | 管志斌 张丽霞 宋美芳 李海涛 张忠廉 |
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| 作者单位： | 中国医学科学院北京协和医学院药用植物研究所云南分所,云南景洪,666100 |
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| 基金项目： | 中央级公益性科研院所基本科研业务专项(YZYN-10-02);西双版纳州三项经费专项(201107) |
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| 摘 要： | 目的:分析常用傣药"傣百解"原植物等9种云南产牛奶菜属植物种间亲缘关系。方法:应用ISSR分子标记技术。结果:从60个ISSR引物中筛选出25个引物用于试验,共扩增出391个条带,每个引物检测到的位点为8～21,平均每个引物扩增出15.6个位点;种间遗传相似系数(GS)在0.667 5～0.821 0;9种牛奶菜属植物间,傣百解Marsdenia auricularis sp.nov.ined.为通光散M.tenacissima的近缘种,云南牛奶菜M.balansae与海枫屯M.officinalis间的亲缘关系最近。结论:ISSR结果支持傣百解为牛奶菜属植物,且与通光散近缘,牛奶菜属多种民族药和民间药值得深入研究。
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| 关 键 词： | 牛奶菜 傣百解 ISSR 亲缘关系 |
| 收稿时间： | 2012-04-16 |
Genetic relationship on several medicinal plants in Marsdenia from Yunnan in ISSR marker |
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| GUAN Zhibin,ZHANG Lixi,SONG Meifang,LI Haitao and ZHANG Zhonglian. Genetic relationship on several medicinal plants in Marsdenia from Yunnan in ISSR marker[J]. China Journal of Chinese Materia Medica, 2012, 37(11): 1550-1552 |
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| Authors: | GUAN Zhibin ZHANG Lixi SONG Meifang LI Haitao ZHANG Zhonglian |
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| Affiliation: | Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Jinghong 666100, China;Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Jinghong 666100, China;Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Jinghong 666100, China;Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Jinghong 666100, China;Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Jinghong 666100, China |
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| Abstract: | Objective: To analyze the genetic relationship of 9 Marsdenia species from Yunnan, especially the traditional Dai medicine "Dai Bai Jie" (M. auricularis). Method: Applying the inter-simple sequence repeats (ISSR) markers technology. Result: Twenty-five primers were screened out of 60 ISSR random primers and produced 391 bands totally, every primer produce 8-21 bands and the mean number was 15.6. The range of the GS(genetic similarity) value was 0.667 5-0.821 0. In 9 Marsdenia species, M. auricularis is a relative of M. tenacissima.M. balansae and M. officinalis have the closest genetic relationship. Conclusion: It is supported by ISSR that the M. auricularis which is sib species of M. tenacissima, and the folk medicine of Marsdenia are worthy deep investigation and study. |
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| Keywords: | Marsdenia Marsdenia auricularis ISSR genetic relationship |
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