基因芯片检测健侧颈7移位术治疗小鼠全臂丛神经损伤后脊髓基因的表达变化

 目的 检测健侧颈₇神经根移位术后脊髓基因表达的变化，探讨神经根移位术后脊髓可塑性形成的机制。方法 30只雄性C57BC/6小鼠，体重20 g，随机分为实验组与对照组，每组15只。实验组又分为3个亚组：损伤即刻修复3个月组，损伤3个月、修复3个月组，损伤1个月、修复6个月组，每组5只。按照上述分组在制作全臂丛损伤模型后行健侧颈₇移位术，于不同时间点取材应用BiostarM-140s基因芯片检测小鼠脊髓组织基因表达的变化，并与对照组进行比较。结果 损伤即刻修复3个月组，损伤3个月、修复3个月组，损伤1个月、修复6个月组脊髓组织分别有55，6和118个基因发生表达水平的改变。其中编码免疫球蛋白重链、chitinase 3的基因在损伤即刻修复3个月组和损伤1个月、修复6个月组均表达增加。编码血管紧张素、collagen、myosin的基因在损伤即刻修复3个月组表达减少，而在损伤1个月、修复6个月组表达增加。突触蛋白synaptotagmin和synaptobrevin在损伤3个月、修复3个月组基因表达改变不明显，而在损伤1个月、修复6个月组表达明显升高。结论 健侧颈₇神经根移位术能引起脊髓组织基因表达水平发生变化，这种变化呈时间依赖性。免疫应答发生于术后各个阶段，而突触可塑性的形成主要发生在术后6个月。

Gene expression profiling of mice spinal cord after contralateral C7 nerve root transfer by microarray analysis

Objective To explore gene expression profile of the spinal cord after contralateral C₇ nerve root transfer, as well as the mechanism of neural plasticity. Methods Thirty male C57BC/6 mice were randomly divided into experiment group and control group with 15 in each. Experiment group was randomly divided into 3 subgroups with 5 in each, which is subgroup 1 (three months after contralateral C₇ nerve root transfer treating immediate brachial plexus root avulsion), subgroup 2 (three months after contralateral C₇ nerve root transfer treating 3 months brachial plexus root avulsion), and subgroup 3 (one month after contralateral C₇ nerve root transfer treating 6 months brachial plexus root avulsion), respectively. A model of contrallateral C₇ nerve root transfer treating left total brachial plexus root avulsion was established in experiment group. We took advantage of BiostarM-140s microarray analysis to detect the differential expression of genes in spinal cord after contralateral C₇ nerve root transfer. Results Contralateral C₇ nerve root transfer could lead to changes in gene expression in spinal cord. There were 55, 6 and 118 differential expressed genes in subgroup 1, 2, 3 respectively. Among them, genes encoding for immunoglobulin heavy chain, chitinase 3 were decreased in sungroup 1 or 3. Genes encoding for angiopoietin, collagen and myotin were deceased in subgroup 1 while increased in subgroup 3. Intriguingly, synaptic proteins like synaptotagmin and synaptobrevin were up-regulated in subgroup 3, whereas no significant changes were found in sungroup 1 and 2. Conclusions The data indicate that changes in gene expression in spinal cord are induced by(nerve root transfer, the change displayed is time-dependent. Immune response is implicated in the whole stage of post-nerve root transfer. Synaptic plasticity occurrs mainly in 6 months.