体外培养的角膜内皮细胞移植研究新进展

随着组织细胞培养方法的不断完善,大量研究表明,人类及灵长目动物的角膜内皮细胞(corneal endothelial cell,CEC)可以在组织培养的条件下生长并形成单层,其细胞形态与活体人CEC类似,这为采用培养的CEC进行移植以恢复损伤的内皮功能提供了技术支持。本文就有关CEC体外培养、载体选择以及移植方法的最新进展进行综述,对目前仍需解决的问题和前景进行展望。

Research advances on transplantation of corneal endotheIial cells cultured in vitro

With the development of tissue cell cultural methods,human corneal endothelial cells(CEC)and some other Primates animal CEC can be harvested as a monolayer from culture supports,and its phenotype and cell activities is similar to human CEC.These data suggests that a well-organized and functional CEC monolayer can feasibly be used as tissue equivalents for replacing compromised endothelium.This article reviews CEC cultivation,selection of different cell seeding and cultivating carriers and progress of monolayer cell transplantation that makes a prospect to the future and the questions needed to answer.