Neuropeptide-Y immunoreactivity in the pilocarpine model of temporal lobe epilepsy |
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Authors: | Lurton Dominique Cavalheiro Esper A |
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Institution: | (1) Neurologia Experimental, Escola Paulista de Medicina-UNIFESP, S?o Paulo, SP, Brazil, BR;(2) Laboratoire de Neurobiologie et Neuro-Imagerie Expérimentales, Université Victor Ségalen–Bordeaux II, 146 rue Léo Saignat, F-33076 Bordeaux-Cédex, France Tel.: +33–5–57571552, Fax: +33–5–56515248, e-mail: 100646.3446@compuserve.com, FR |
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Abstract: | Neuropeptide-Y (NPY) is expressed by granule cells and mossy fibres of the hippocampal dentate gyrus during experimental
temporal lobe epilepsy (TLE). This expression may represent an endogenous damping mechanism since NPY has been shown to block
seizure-like events following high-frequency stimulation in hippocampal slices. The pilocarpine (PILO) model of epilepsy is
characterized by an acute period of status epilepticus followed by spontaneous recurrent seizures and related brain damage.
We report peroxidase-antiperoxidase immunostaining for NPY in several brain regions in this model. PILO-injected animals exhibited
NPY immunoreactivity in the region of the mossy fibre terminals, in the dentate gyrus inner molecular layer and, in a few
cases, within presumed granule cells. NPY immunoreactivity was also dramatically changed in the entorhinal cortex, amygdala
and sensorimotor areas. In addition, PILO injected animals exhibited a reduction in the number of NPY-immunoreactive interneurons
compared with controls. The results demonstrate that changes in NPY expression, including expression in the granule cells
and mossy fibres and the loss of vulnerable NPY neurons, are present in the PILO model of TLE. However, the significance of
this changed synthesis of NPY remains to be determined.
Received: 19 August 1996 / Accepted: 21 March 1997 |
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Keywords: | Pilocarpine NPY Hippocampus Epilepsy Immunohistochemistry Rat |
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