脑组织提取液促进体外培养神经干细胞增殖

目的： 寻找特异性的具有促神经干细胞分裂增殖作用的物质，为神经系统发育研究和神经系统疾病包括脑退行性疾病的治疗研究提供新资料。 方法： 制备新生鼠的前脑、中脑、后脑及小脑组织提取液，体外培养新生鼠神经干细胞，观察神经球的生长情况，采用MTT细胞活性检测法和特异性蛋白质免疫细胞化学染色鉴定神经干细胞的增殖能力。 结果： 中脑和小脑的提取液加入后有大量神经球生成，神经干细胞的特异性蛋白nestin免疫细胞化学染色呈阳性。 结论： 中脑和小脑的提取液可促进体外培养的神经干细胞增殖分裂，且呈一定量效关系。

Proliferative effect of neonatal mouse brain extracts on neural stem cells in vitro

AIM: To discover specific neural stem cell (NSC) proliferation-promoting factor, which will contribute to study on development of nervous system and treatment of nervous system diseases. METHODS: The extracts of forebrain, midbrain,afterbrain and cerebellum of neonatal mice were prepared, and the NSCs of newborn mice were cultured in vitro. Neurospheres were observed, immunocytochemical staining of characteristic protein, nestin, and MTT assay were performed to identify NSCs and their proliferative properties. RESULTS: A great deal of neurospheres were formed in the presence of the extracts of afterbrain and cerebellum, which were positive for characteristic protein (Nestin) of NSC showed by immunocytochemical staining. CONCLUSION: The afterbrain and cerebellum extracts can increase the total number of NSCs isolated from newborn mice in vitro in a dose-dependent manner.