慢性砷中毒大鼠肝脏血清酶活性变化分析

目的 探讨慢性砷中毒大鼠肝脏和肝线粒体损伤相关血清酶活性的变化,为揭示砷对机体毒性作用的机制及选择慢性砷中毒早期诊断酶学指标提供资料。方法 选用Wistar大鼠,经饮水给予75 mg/L三氧化二砷6个月。处死动物6个月,用差速离心法提取大鼠肝脏线粒体,用分光光度法测定肝脏线粒体呼吸酶及血清酶活性。结果 砷中毒大鼠肝脏线粒体细胞色素C氧化酶和三磷酸腺苷(ATP)酶活性下降;血清谷氨酸脱氢酶、鸟氨酸氨基甲酰转移酶、精氨酸酶、腺苷脱氨酶、谷胱甘肽硫转酶活性升高。结论 慢性砷中毒能够导致肝线粒体细胞色素C氧化酶和ATP酶的活性下降。血清中与肝细胞及线粒体功能有关的血清酶活性升高,进一步证实了砷中毒对肝细胞及其线粒体的损害作用。上述5种血清酶的测定,对于慢性砷中毒的辅助诊断可能具有一定的价值。

Study on change of serum enzymes activity in chronic arsenism rats

Objective To explore the pathogenesis of arsenism and search the useful clinical enzymological indices of arsenism, the changes of the serum enzymes correlating to the damage of hepatic cells and hepatic mitoehondria were studied in chronic arsenism rats. Methods 75 mg/L arsenic trioxide was administered to Wistar rats through drinking water for 6 months. Hepatic mitochondria was extracted by differential velocity centrifugation. The activities of respiratory enzymes of hepatic mitoehondria and the serum enzymes were determined by spectrophotometer. Results The activities of hepatic mitoehondria cytoehrom C oxidase and ATPase were decreased, and the serum activities of glutammate dehydrogenase, ornithine carbamoyl transferase, adenosine deaminase, argininase, glutathione-S-tranferase increased in arsenism rats. Conclusion The chronic arsenism could result in the activity decline of the mitochondria eytochrome C oxidase and ATPase. The incremental activities of serum enzymes which are relative to the fumction of hepatic cell and mitochondria further demonstrate the damage to hepatic cell and its mitochondria from arsenism. The determinations of the activities of five enzymes above are with some values to the assistant diagnoses of chronic arsenism.