Different Ca2+ dynamics between isolated hippocampal pyramidal cells and dentate granule cells |
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Authors: | Baba Atsushi Yamada Maki K Nishiyama Nobuyoshi Matsuki Norio Ikegaya Yuji |
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Institution: | (1) Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, 113-0033, Japan |
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Abstract: | The hippocampal formation contains a variety of neuronal types. The principal neurons are granule cells in the dentate gyrus and pyramidal cells in Ammon's horn. These two neuron types show distinct cell morphology and display a different vulnerability to ischemic injury or various neurotoxins. In order to illustrate the difference in the pathophysiological properties of these neurons, we established a method for separately culturing granule cells and pyramidal cells. They were prepared from the dentate gyrus and Ammon's horn of 3-day-old Wistar rat pups and maintained for 7–9 days in culture. After transient exposure to N-methyl-D-aspartate or glutamate, both the cultured neuron populations displayed somatic Ca2+ transients with similar amplitudes, but the subsequent recovery to baseline was about twice as fast in granule cells than in pyramidal cells. Similar results were obtained for K+ depolarization-induced Ca2+ elevation, suggesting that the relatively rapid Ca2+ clearance in granule cells is independent of Ca2+ influx pathways. The present study provides the first evidence for a difference in Ca2+ dynamics and homeostasis between granule and pyramidal cells and may represent a cellular basis for the differential vulnerability of hippocampal neurons. |
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