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Helicobacter pylori lipopolysaccharide-provoked injury to rat gastroduodenal microvasculature involves inducible nitric oxide synthase |
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| Authors: | J zsef Kiss, Dominique Lamarque, Anthony P. Moran, J zsef Pozs r, va Morschl, Ferenc L szl ,Brendan J. R. Whittle |
| Affiliation: | a First Department of Medicine, University Medical School of Szeged, Hungary b Henri Mondor Hospital, INSERM U99, Creteil, France c Department of Microbiology, National University of Ireland, Galway, Ireland d Second Department of Medicine, St. Imre Hospital, Budapest, Hungary e Institute of Experimental Medicine, Budapest, Hungary f The William Harvey Research Institute, St. Bartholomew's and Royal London School of Medicine, Charterhouse Square, London EC1M 6BQ, UK |
| Abstract: | The actions of a purified Helicobacter pylori lipopolysaccharide (3 mg kg−1, i.v.) on rat gastric antral and duodenal microvascular integrity (determined as radiolabelled albumin leakage) and the expression of the inducible nitric oxide (NO) synthase (iNOS; assessed by the citrulline assay) were investigated 4 h after challenge. Significant increases of albumin leakage and expression of iNOS in both antral and duodenal tissues were observed following challenge. Concurrent administration of the selective iNOS inhibitor, 1400W (N-(8-(aminomethyl)benzyl)-acetamidine; 0.2–1 mg kg−1, s.c.), with lipopolysaccharide, caused a dose-dependent attenuation of the gastric and duodenal albumin leakage. Thus, H. pylori lipopolysaccharide can initiate the expression of iNOS in the stomach and duodenum following systemic challenge, which can provoke gastroduodenal microvascular dysfunction. |
| Keywords: | Helicobacter pylori Lipopolysaccharide Gastritis Duodenum Inflammation Nitric oxide (NO) synthase inhibitor Nitric oxide (NO) synthase, inducible |
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