| 小鼠乳腺癌模型中CD4~+CD25~(bright)CCR6~+Treg的检测及其意义 |
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| 引用本文： | 徐林,徐薇,蒋正刚,熊思东.小鼠乳腺癌模型中CD4~+CD25~(bright)CCR6~+Treg的检测及其意义[J].现代免疫学,2010(1). |
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| 作者姓名： | 徐林 徐薇 蒋正刚 熊思东 |
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| 作者单位： | 复旦大学免疫生物学研究所上海医学院免疫学系; |
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| 基金项目： | 国家973资助项目(2007CB512401); 上海市医学领军人才基金资助项目(LJ06011) |
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| 摘 要： | 为检测CD4+CD25brightCCR6+Treg在小鼠乳腺癌实验动物模型中的分布,并探讨其意义。采用FACS检测正常小鼠和4T1荷瘤小鼠中CD4+CD25brightTreg的记忆分子CCR6的表达水平,同时检测CD4+CD25brightTreg的CCR6+和CCR6-两个亚群的Foxp3表达情况;用增殖抑制实验观察了两个亚群分别对CD4+CD25-T细胞增殖的抑制作用;用FACS检测CD4+CD25brightCCR6+Treg在正常小鼠和4T1荷瘤小鼠中PBMC、LN和TIL中的分布情况。结果:4T1荷瘤小鼠中CD4+CD25brightTreg的记忆分子CCR6的表达水平较正常小鼠增加;CD4+CD25brightTreg的CCR6+和CCR6-两个亚群均高表达Foxp3,均能在体外有效抑制CD4+CD25-T细胞的增殖;与正常对照相比,CD4+CD25brightCCR6+Treg在4T1荷瘤模型的引流淋巴结中比例明显增加,并在肿瘤局部存在显著的富集。上述结果提示在肿瘤免疫中存在CD4+CD25brightCCR6+Treg,其具有效应/记忆样表型,并在肿瘤局部有明显的富集,这可能是肿瘤长期免疫逃逸的重要机制。
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| 关 键 词： | CD4+CD25+Treg 免疫逃逸 Foxp3 CCR6 |
The detection and its significance of CD4~+CD25~(bright)CCR6~+ regulatory T cells in murine mammary carcinoma model |
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| XU Lin,XU Wei,JIANG Zheng-gang,XIONG Si-dong.The detection and its significance of CD4~+CD25~(bright)CCR6~+ regulatory T cells in murine mammary carcinoma model[J].Current Immunology,2010(1). |
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| Authors: | XU Lin XU Wei JIANG Zheng-gang XIONG Si-dong |
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| Institution: | XU Lin,XU Wei,JIANG Zheng-gang,XIONG Si-dong(Institute for Immunobiology & Department of Immunology,Shanghai Medical College,Fudan University,Shanghai 200032,China) |
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| Abstract: | To investigate the distribution of CD4+CD25brightCCR6+ regulatory T cells in experimental murine mammary carcinoma model and to explore its significance.An experimental animal model of mouse mammary carcinoma was established by subcutaneous injection of 4T1 cells.The expression of CCR6 on CD4+CD25bright regulatory T cells from nomal BALB/c mice and 4T1 bearing syngeniec mice were analyzed by FACS.The expression level of Foxp3 on CCR6+ and CCR6-subsets of CD4+CD25bright regulatory T cells were analyzed by FA... |
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| Keywords: | CD4+CD25+ regulatory T cells immune escape Foxp3 CCR6 |
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