| HPLC法测定肋柱花中木犀草素的含量 |
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| 引用本文： | 王秀梅,巴根那,白明纲. HPLC法测定肋柱花中木犀草素的含量[J]. 中国民族医药杂志, 2009, 15(1): 38-39 |
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| 作者姓名： | 王秀梅 巴根那 白明纲 |
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| 作者单位： | 内蒙古民族大学蒙医药学院,通辽,028000 |
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| 摘 要： | 目的：建立高效液相色谱（HVLC）法测定肋柱花中木犀草素含量的方法。方法：采用高效液相色谱法。色谱柱：Dia—monsilC18（250mm×4．6mm，5μm）；流动相：甲醇-0．4％磷酸溶液（55：45）；流速：1．0ml·min^-1；检测波长：350nm；柱温：35℃。结果：木犀草素对照品在0．052μg-0．312μg范围内，与峰面积具有良好的线性关系，r=0．9999；平均回收率为98．13％、RSD为1．04％（n=6）。结论：该方法简便、快捷、准确、重复性好，可用于肋柱花中木犀草素的含量测定。
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| 关 键 词： | 蒙药 肋柱花 木犀草素 HPLC |
Determination of luteolin in Herba Lomatogonii by HPLC |
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| WANG Xiu-Mei,Bagenna,BAI Ming-gang. Determination of luteolin in Herba Lomatogonii by HPLC[J]. Journal of Medicine & Pharmacy of Chinese Minorities, 2009, 15(1): 38-39 |
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| Authors: | WANG Xiu-Mei Bagenna BAI Ming-gang |
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| Affiliation: | WANG Xiu- Mei ,Bagerma ,BAI Ming- gang (College of Mongolian Medicine and pharmacology, Inner Mongolia University for the Nationalities, Tongliao 028000) |
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| Abstract: | objective： To establish a method for the determination of luteolin in Mongolian medicine Lomatonium rataturn （L.） Fries by HPLC. Method： Determining the luteolin in Mongolian medicine Lomatonium rotatum （L.） Fries by ttPLC on Diamonsil C18（250mm×4.6mm,5μm） with Methyl- 0.4% Phosphoric acid （55：45 v/v） as a mobile phase and the flow rate is 1.0 ml·min^-1, the detective wavelength is 350 nm, the column temperature is 35℃ .Resuits ： The good linear relationship of Luteolin is abtained between 0.052μg - 0. 312μg, r = 10000. The average recovery is 98.13%, RSD = 1.04% （n = 6）. Conclusion： The method is simple, rapid, accurate, good re - appearance, and can be used for determining the content of Luteolin in Mongolian medicine Herba Lomatogonii. |
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| Keywords: | HPLE |
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