| 一组抗人肺癌单克隆抗体的制备及初步鉴定 |
| |
| 引用本文： | 王登顺,袁玫,史景泉. 一组抗人肺癌单克隆抗体的制备及初步鉴定[J]. 细胞与分子免疫学杂志, 1992, 0(2) |
| |
| 作者姓名： | 王登顺 袁玫 史景泉 |
| |
| 作者单位： | 解放军514医院实验医学科，解放军总医院肿瘤单克隆室，第三军医大学病理学教研室 北京 100101 |
| |
| 摘 要： | 本文报告用人肺腺癌细胞株A549细胞为免疫原,用常规细胞融合技术获得了5株抗人肺癌单克隆抗体WLA-1B4、WLA-2D1、WLA-1G7、WLA-1G9和WLA-2C4。前4株均为IgM型,后1株为IgG1型。添加试验表明WLA-2D1和WLA-1G9识别同一抗原决定簇,WLA-2C4,WLA-1B4识别不同的抗原决定簇。ABC法免疫组织化学研究表明,5株单抗与肺癌组织均呈阳性反应,而与正常组织交叉反应较少。
|
| 关 键 词： | 抗体 单克隆 肺癌 制备 |
Preparation and preliminary characterization of five monoclonal antibodies to lung carcinoma |
| |
| Wang Dengshun et al. Preparation and preliminary characterization of five monoclonal antibodies to lung carcinoma[J]. Chinese journal of cellular and molecular immunology, 1992, 0(2) |
| |
| Authors: | Wang Dengshun et al |
| |
| Abstract: | Five monoclonal antibodies (WLA-2C4, WLA-1B4, WLA-2D1 WLA-1G7 and WLA-1G9 ) to human lung carcinoma vere prepared by fusion of spleen cells from BALB/c mice immunized with cultured intact cells of the cell line A549. Additive tests showed that McAbs WLA-1B4 and WLA-2C4 identified different epitopes of antigen(s) whereas the epitopes recognized by WLA-2D1 and WLAlG9 were identical. The epitope recognized by WLA-2C4 was protein' in nature and the epitopes recognized by the other tour McAbs were carbohydrates. Immunohistochemical study indicated that the five McAbs reacted with paraffin-embedded tissues of human lung carcinoma and, with the exception of WLA-2C4, four McAbs demonstrated mild to moderate cross-reactivity with normal adult tracheobronchial tissucs and alveolar epithelium. These McAbs are still under further investigation. |
| |
| Keywords: | antibody monoclonal neoplasms lung preparation |
| 本文献已被 CNKI 等数据库收录！ |
|
