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| Localization of P2X7 Receptor Immunoreactivity in the Dorsal Root Ganglia of Guinea Pig | |
| 作者姓名： | 柏学工 蒋铃 向正华 |
| 作者单位： | Department of Neurology，Xiangfan Hospital，Tongji Medical College，Huazhong University of Science and Technology，Xiangfan 441021，China，Shanghai University of Science and Technology，Shanghai 200093，China，Department of Biochemistry and Molecular Biology，Second Military Medical University，Shanghai 200433，China |
| 摘 要： | Adenosine5'triphosphate(ATP)canbere leasedbyavarietyofcellsincludingcentralandpe ripheralneuronsandglialcellsandelicitfastneu rotransmitter likeresponsesbyactivatingmembersoftheP2Xreceptorfamily.SevenP2Xreceptorsubtypesorsubunitshavebeencloned1].Homo and/orheteromultimericexpressionofthecorre spondingproteinsformsfunctionalreceptorsthatoperateasligand gatedionchannelswithavarietyofkinetic,pharmacologicalandcationicselectivity(includingcalciumpermeability).TheP2X7recep torhasseveralunusual… |
| 关 键 词： | P2X7受体 免疫反应 脊髓神经 中枢神经 几内亚猪 动物实验 细胞化学 |
| 收稿时间： | 2005-01-20 |
Localization of P2X7 Receptor Immunoreactivity in the Dorsal Root Ganglia of Guinea Pig |
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| BAI Xuegong,JIANG Ling,XIANG Zhenghua.Localization of P2X7 Receptor Immunoreactivity in the Dorsal Root Ganglia of Guinea Pig[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2005,25(4):371-374,396. | |
| Authors: | BAI Xuegong JIANG Ling XIANG Zhenghua |
| Institution: | 1. Department of Neurology, Xiangfan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Xiangfan 441021, China 2. Shanghai University of Science and Technology, Shanghai 200093, China 3. Department of Biochemistry and Molecular Biology, Second Military Medical University, Shanghai 200433, China |
| Abstract: | The P2X7 receptor mRNA and proteins in guinea-pig dorsal root ganglia (DRG) were studied by using RT-PCR and immunohistochemistry. The co-localization of P2X7 receptor with four cytochemical markers, the neurofilament protein NF200, S100, substance P and isolectin B4 (IB4) binding glyco-conjugates, were also examined. It was found that P2X7 receptor immunoreactivity (P2X7 R-IR) was present mostly in large- and medium-sized DRG neurons (62% +/- 9% and 36% +/- 6% respectively in all P2X7 R-IR neurons). All the P2X7R-IR neurons were also NF200 and S100 immunopositive. However, in a small number of NF200 or S100 immunopositive neurons no P2X7R-IR was detectable. All the IB4-positive or substance P-immunopositive neurons had no P2X7R-IR. These results demonstrate that P2X7 receptors are expressed in a large subpopulation of DRG neurons and they may play a role in the transduction of specific peripheral sensory signals. |
| Keywords: | P2X7 receptor ATP receptor purinoreceptor dorsal root ganglion NF200 S100 isolectin B4 substance P |
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