远志皂苷元对AD模型小鼠学习记忆能力的影响及其机制研究 |
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引用本文: | 蒋辉,徐运,姜亚军. 远志皂苷元对AD模型小鼠学习记忆能力的影响及其机制研究[J]. 现代中西医结合杂志, 2013, 22(11): 1153-1155 |
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作者姓名: | 蒋辉 徐运 姜亚军 |
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作者单位: | 蒋辉 (江苏省中医院,江苏,南京,210029);徐运 (江苏省中医院,江苏,南京,210029); 姜亚军 (江苏省中医院,江苏,南京,210029); |
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摘 要: | 目的观察远志皂苷元对阿尔茨海默病(AD)模型小鼠的学习记忆能力的影响,并初步探讨其作用机制。方法采用对小鼠海马立体定向注射Aβ1-42的方法建立AD动物模型,并随机分为假手术组,模型组,低剂量、中剂量、高剂量治疗组,连续治疗30 d后,运用Morris水迷宫检测各组小鼠学习记忆能力,随后进行活性氧簇(ROS)、8-羟基脱氧鸟苷(8-OHdG)、3-硝基酪氨酸(3-NT)等氧化应激指标检测。结果与模型组相比,各治疗组小鼠的潜伏期明显缩短,穿台次数明显增多,海马组织的ROS、8-OHdG、3-NT含量均有明显减少(P均<0.05)。结论远志皂苷元能明显改善AD模型小鼠的学习记忆能力,其机制可能与抗氧化应激作用有关。
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关 键 词: | 远志皂苷元 阿尔茨海默病 学习记忆 氧化应激 |
Effect of Senegenin on learning and memory ability of AD model mice and its mechanism |
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Abstract: | Objective It is to observe the effect of Senegenin on learning and memory abilities of Alzheimer's disease(AD) model mice,and to explore its mechanism.Methods Using mouse hippocampus stereotactic injection of Aβ(1-42) method to establish animal models of(AD),and the models were randomly divided into sham group,model group,low-dose,medium-dose and high-dose treatment groups,after continuous treatment of 30 d using Morris water maze to test learning and memory abilities of the mice in each group,then oxidative stress indicators such as reactive oxygen clusters(ROS),8-hydroxy-deoxy-guanosine(8-OHdG),3-nitrotyrosine(3-NT) were determined.Results Compared with model group,the incubation period of the mice in treatment groups was significantly decreased,wear station was significantly increased,and the contents of hippocampus ROS,8-OHdG,3-NT were significantly reduced(all P<0.05).Conclusion Senegenin can significantly improve learning and memory abilities of AD model mice,the mechanism may be related to its effect of anti-oxidative stress. |
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Keywords: | Senegenin Alzheimer's disease learning and memory oxidative stress |
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