鱼粉代替谷氨酸生产麦角新碱的研究

在以前工作的基础上,我们进一步采用鲫鱼粉代替谷氨酸生产麦角新碱取得了比较好的效果。通过试验证明,鲫鱼粉不仅可代替谷氨酸作为氮源,并能促进麦角新碱的形成,其麦角新碱含量比对照高近一倍,且其来源容易,价格低廉。麦角菌在200l发酵罐内培养,从第四天开始产生麦角碱,发酵至11天麦角碱含量已达最高峰。在发酵过滤液中麦角总生物碱及麦角新碱含量分别为0.0203%及0.0178%,而在菌丝体中分别达到0.1609%及0.0495%。

THE REPLACEMENT OF GLUTAMIC ACID BY FISH MEAL FOR THE PRODUCTION OF ERGOMETRINE BY CLAVICEPS MICROCEPHALA IN SUBMERGED CULTURE

Glutamic acid has been conventionally used as a nitrogen source for the medium in studying ergometrine production by ergot organisms in submerged culture. The high price of glutamic acid, however, is a serious disadvantage for large scale production of ergot alkaloids. Thus we attempted to select several cheaper and easily obtainable natural subatances for the substitution of glutamic acid.It was shown through our experiments that crucian carp (Carassius suratus) meal and silver carp (Hypaphthalmichths molitrix) meal were most effective, resulting in pronounced increase of ergot alkaloid production as compared with glutamic acid.Different concentration of crucian carp meal were tested. It was found that the 4% meal gave best results, with total alkaloid and ergometrine production of 0.0185% and 0.0115% respestively.Using crucian carp meal as the nitrogen source in 200-liter submerged fermentors, the ergot orgamism (Strain Ge-3-45) started to produce ergot alkaloid on the 4th day. The amount of the total alkaloid and ergometrine reached maximum, 0.2034% and 0.01781% in filtrate(0.1609% and 0.0495% in mycelium) respectively, after about 11 days fermentation.It may be concluded that crucian carp meal is a Letter nitrogen source than glutamic acid for the production of ergot alkaloid in submerged culture, not only for its greater enchancement of the production of ergometrine but also for its availability and inexpensiveness.