功能性电刺激对急性脑梗死大鼠运动功能和缺血半影区与镜区突触素表达的影响

目的：观察功能性电刺激（FES）对急性脑梗死大鼠运动功能和缺血半影区与镜区突触素（SYN）表达的影响。方法：采用Longa线栓法制作大脑中动脉栓塞(MCAO)模型，MCAO造模步骤结束后，在大鼠偏瘫侧上肢埋置金属导线连接FES治疗仪。将大鼠随机分为3组：假手术组、安慰电刺激组和FES组，每组又分为0d、3d、7d、14d 4个亚组，每亚组6只。FES在术后3d开始，使大鼠偏瘫上肢产生伸腕和伸指动作，每天1次，每次10min。在FES刺激前后的各个时间点进行网屏实验以观察各组的运动功能变化；应用Western-blot技术检测缺血半影区和镜区SYN蛋白表达。结果：FES组在刺激7d和14d后运动功能较安慰电刺激组明显改善(P<0.05)。脑缺血半影区在刺激3d、7d、14d后、镜区在刺激7d、14d后，FES刺激组较安慰电刺激组SYN蛋白表达水平明显增高(P<0.05)。结论：FES可以改善急性脑梗死大鼠的运动功能，并增强脑梗死周围缺血半影区和镜区SYN的蛋白表达，即增强脑的可塑性，但半影区的可塑性出现早于镜区，且表达强于镜区。

Effects of functional electrical stimulation on motor function and expression of synaptophysin in penumbra field and contralesional field of rats with acute cerebral infarction

Objective :To observe the effects of functional electrical stimulation (FES) on motor function and expres-sion of synaptophysin (SYN) in penumbra field and contralesional field of rats with acute cerebral infarction. Method :Rat model of middle cerebral artery occlusion (MCAO) was established with Longa's method of intraluminal filament occlusion. Wires were implanted on the hemiplegic upper limbs for connecting with FES instrument. Rats were randomly divided into FES group, FES placebo group and sham operation group. In each group, rats were randomly allocated imo baseline, 3d, 7d and 14d subgroups (6/subgroup). FES treatment was carried out 3d after MCAO operation to cause extension of wrist and digitals of hemiplegic upper limb. Scores of screen test was as-sessed at each time point mentioned above to observe the motor function. Expression levels of SYN was assessed by Western blot technique. Result:After treatment for 7d and 14d, the motor function of FES group improved sig-nificantly compared with that of FES placebo group (P<0.05). Compared with that of FES placebo group, SYN pro-tein expression levels in FES group were higher at the 3rd d, 7th d and 14th d treatment time points in penumbra field and at the 7th d and 14th d treatment points in contralesional field (P<0.05). Conclusion:FES treatment can improve motor function and enhance the expression of SYN in penumbra field and contralesional field of rats with acute cerebral infarction. FES can enhance the cerebral plasticity.