氟致人胎肝细胞氧化应激与细胞凋亡关系的研究

目的：研究氟致人胎肝细胞氧化应激和细胞凋亡之间的关系。方法：体外培养的人胎肝细胞接触不同浓度的氟化钠24h后，检测人胎肝细胞凋亡百分率、细胞周期构成比、脂质过氧化物(LPO)、还原型谷胱甘肽(GSH)和培养液中LPO水平以及乳酸脱氢酶（LDH)、丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)的活性。结果：氟染毒组培养液中LDH和AST活性均显著高于对照组；细胞内和上清液中LPO水平升高，细胞内GSH则明显下降，并且二者均与氟浓度呈现明显的剂量一效应关系；氟可使人胎肝细胞凋亡百分率明显升高，S期细胞数增多。结论：氟可致人胎肝细胞氧化应激，诱导细胞凋亡，一定浓度范围内的氟所诱导的氧化应激与细胞凋亡之间呈现明显的正相关关系。

Relationship between oxidative stress and apoptosis induced by fluoride in hmnan embryo hepatocytes

Objective To explore the relationship between oxidative stress and apoptosis in human embryo hepatocytes induced by fluoride. Methods The apoptosis, cell cycle, lipid peroxidation (LPO) level and reduced glutathione (GSH) content in cells, LPO level, activities of lactic dehydrogenase (LDH), alkaline aminotransferase (ALT) and aspartate aminotransferase (AST) in cell culture supernatants were investigated after the cells were incubated with sodium fluoride for about 24 hours. Results The activities of LDH and AST in supernatants in the three fluoride treated groups were higher than those of the control group. Fluoride increased LPO levels in cells and supernatants, and reduced cellular level of GSH, which showed dose-effect relationship between level of fluoride and levels of LPO and GSH. Fluoride enhanced the percentage of human embryo hepatocytes apoptosis bodies and increased the number of cell in S phase. Conclusions Fluoride can activate oxidative stress and induce apoptosis of human embryo hepatocytes. Furthermore, there exists a significant positive correlation between oxidative stress and apoptosis induced by fluoride in certain level range.