大鼠失血性休克再灌注肾损伤的实验研究

目的 探讨大鼠失血性休克再灌注肾损伤(HS－RRI)的特点。方法制备大鼠HS－RRI模型,观察各组肾组织病理形态学改变和树突状细胞(DC)的分布特点及二者的相关性。结果休克末期,大鼠平均失血量占总血量的60.42%。休克组的肾组织损伤重于复苏组,但DC的数量少于复苏组;再灌注后的肾组织病理形态学改变和间质DC的数量均以24 h最明显;间质DC的变化与组织损伤呈正相关(P＜0.01)。结论HS－RRI以复苏后24h的肾组织损伤最明显、间质DC分布最广 : DC参与肾组织损伤。

Renal ischemic-reperfusion injury following hemorrhagic shock in rats: an experimental study

Objective To understand the features of renal ischemic-reperfusion injury after hemorrhagic shock in rats. Meth-ods Models of hemorrhagic shock were established in 36 Sprague-Dawley rats that were divided into 6 groups (with 6 rats ineach group), 5 groups of which received subsequent resuscitation measures. Another 6 untreated normal rats served as normalcontrol. Renal pathomorphoiogy and the distribution of dendritic cells (DCs) were observed to determine their correiation inthe resuscitation groups (at 3, 6, 12, 24 and 48 h respectively after resuscitation), the control and shock groups. Results Theblood loss of the rats averaged 60.42% of the total blood at the end of hemorrhagic shock. More severe pathological changeswere observed in the rats with shock but without receiving resuscitation measures, as compared with the changes in rats withrescuscitation. The rats in shock group had the fewest DC number of all the groups. Among the groups with repeffosion aftershock. the most severe renal pathomorphological changes took place 24 h after the resuscitation when the most significant DCactivation was noted in positive correlation with renal tissue injury (W0.0l). Conclusions Twentyfour hours after repemi-sion, the rats with hemorrhagic shock experience the most severe changes in renaI pathomorphology with the most extensivedistribution of the DCs. indicating that DCs induce renal tissue injury.