| MiR-738通过Wnt信号通路调控斑马鱼心脏发育 |
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| 引用本文： | 施林生,黄建飞,卢辉和,陈小丽,郑扣龙,林刚. MiR-738通过Wnt信号通路调控斑马鱼心脏发育[J]. 南通医学院学报, 2014, 0(1): 8-10,F0002 |
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| 作者姓名： | 施林生 黄建飞 卢辉和 陈小丽 郑扣龙 林刚 |
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| 作者单位： | 江苏省南通市第一人民医院心内科,南通226001 |
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| 基金项目： | 南通市科技计划项目（BK2011057） |
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| 摘 要： | 目的：研究miR-738对模式动物斑马鱼心脏发育的影响。方法：通过全胚胎原位杂交检测miR-738的表达。在斑马鱼受精卵中显微注射miR-738前体来过表达miR-738。通过显微镜成像来检测心脏表型。通过荧光素酶报告基因检测来验证miR-738调控Lefl。结果：MiR-738在30hpf斑马鱼心脏表达。MiR-738过表达导致斑马鱼胚胎心脏显著增大。MiR-738可以抑制Lefl的表达。结论：MiR-738通过Wnt信号通路调控斑马鱼心脏发育。
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| 关 键 词： | miR-738 心脏 Wnt 斑马鱼 发育 |
MiR-738 regulates the zebrafish heart formation through Wnt signaling |
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| SHI Linsheng,HUANG Jianfei,LU Huihe,CHEN Xiaoli,ZHENG Koulong,LIN Gang. MiR-738 regulates the zebrafish heart formation through Wnt signaling[J]. ACTA Academiae Medicinae Nantong, 2014, 0(1): 8-10,F0002 |
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| Authors: | SHI Linsheng HUANG Jianfei LU Huihe CHEN Xiaoli ZHENG Koulong LIN Gang |
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| Affiliation: | (Department of Cardiology, First People＇s Hospital of Nantong, Nantong 226001) |
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| Abstract: | Objective： To examine the function of miR-738 involved in zebrafish heat development. Methods： By microinjec- tion of miR-738 precursor into zebrafish fertilized egg to overexpress miR-738. By microscope imaging to check the heart phe-notype. By using luciferase reporter assay to validate that miR-738 regulates Lefl directly. Results： MiR-738 was detected in zebrafish heart at 30 hpf. Overexpression of miR-738 resulted in the enlargement of zebrafish embryonic heart. MiR-738 in-hibits the Lefl expression. Conclusion： MiR-738 regulates the zebrafish heart formation through Wnt signaling. |
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| Keywords: | miR-738 heart Wnt zebrafish development |
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