| 大肠癌LoVo细胞培养液和裂解液中蛋白质的飞行质谱法比较 |
| |
| 引用本文： | 王秀丽,高春芳,赵光,李冬晖.大肠癌LoVo细胞培养液和裂解液中蛋白质的飞行质谱法比较[J].实用医药杂志(山东),2006,23(11):1344-1346. |
| |
| 作者姓名： | 王秀丽 高春芳 赵光 李冬晖 |
| |
| 作者单位： | 150医院肛肠科,河南洛阳471031 |
| |
| 摘 要： | 目的探讨LoVo细胞培养液、裂解液中蛋白质有无异同。方法应用美国CipherGen公司IMAC3(Immobilized metal affinity capture,金属亲和表面)芯片和蛋白质芯片仪检测LoVo细胞培养液、裂解液进行对比研究。结果LoVo细胞培养液、裂解液所含蛋白质质荷比不同。结论LoVo细胞培养液、裂解液无相同的蛋白质。
|
| 关 键 词： | 蛋白质组学 LoVo细胞 裂解 |
| 修稿时间： | 2006年5月30日 |
Studies on proteomics of LoVo cells suspension and LoVo cells lysates by using SELDI-TOF mass spectrometry |
| |
| WANG Xiu-li,GAO CHun-fang,ZHAO Guang,et al..Studies on proteomics of LoVo cells suspension and LoVo cells lysates by using SELDI-TOF mass spectrometry[J].Practical Journal of Medicine & Pharmacy,2006,23(11):1344-1346. |
| |
| Authors: | WANG Xiu-li GAO CHun-fang ZHAO Guang |
| |
| Institution: | WANG Xiu-li,GAO CHun-fang,ZHAO Guang,et al. Dept. of Coloproctology,the 150th Cenler Hospital of PLA,Luoyang 471031,China |
| |
| Abstract: | Objective To explore the similarity of biomarkers expression between LoVo cells suspension and LoVo cells lysates.Methods LoVo cells suspension and LoVo cells lysates were lesled by IMAC3 chip and proleinchip reader of ciphergen Inc.Results There were no proteins at the same M/Z values in the LoVo cells suspension and LoVo cells lysates.Conclusion There are no similarity of protein between LoVo cells suspension and LoVo cells lysates. |
| |
| Keywords: | Biomarkers LoVo cells Lysates Proleomics |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
