山麦冬总皂苷对局灶性脑缺血损伤的保护及抗凝血作用研究

目的:研究山麦冬总皂苷(TSL)对大鼠局灶性脑缺血损伤的保护及抗凝血作用。方法:采用三氯化铁局部涂抹损伤血管的方法复制大鼠大脑中动脉血栓模型,观察TSL对大鼠行为障碍、脑梗死范围、相关脑区组织病理变化、神经型一氧化氮合酶(nNOS)阳性细胞表达率等指标的影响;采用毛细管法及断尾法观察TSL对小鼠出、凝血时间的影响。结果:10、40mg.kg-1TSL可显著减少大鼠脑梗死范围,改善行为学障碍,降低nNOS阳性细胞表达率;20、60mg.kg-1TSL可使小鼠凝血时间及出血时间显著延长。结论:TSL对大脑中动脉血栓所致局灶性脑缺血损伤具有保护作用,并具有显著的抗凝血作用。

Neuroprotective Effect and Anticoagulation Effect of Total Saponins of Radix Liriopes on Focal Cerebral Ischemia

OBJECTIVE:To investigate the neuropotective effect and anticoagulation effect of total Saponins of Radix Liriopes on focal cerebral ischemia in rats.METHODS:Chemical reagent Fecl3 was locally applied on the injured vessels to establish middle cerebral artery thrombosis model,and the effects of total Saponins of Radix Liriopes on rats' behavioral disturbance,brain infarct size,the histopathological changes in brain and the expression rate of nNOS immunoreactive positive neurons were measured,and the bleeding time and coagulation time were also detected with glass tube method and tail transection method.RESULTS:Due to the use of total Saponin of Radix Liriopes(10 and 40mg· kg-1),the brain infarct size was significantly decreased,the behavioral disturbance were improved and the expression rate of the nNOS immunoreactive positive neurons in rats were decreased.At doses of 20 and 60mg· kg-1,total Saponin of Radix Liriopes significantly prolonged the coagulation time and bleeding time.CONCLUSION:Total Saponin of Radix Liriopes has nuroprotective effect on focal cerebral ischemia induced by middle cerebral artery thrombosis and significant anticoagulation effect.