靶向巨噬细胞表达抗菌肽PR39基因抗胞内结核分枝杆菌的研究

目的:观察靶向巨噬细胞表达抗菌肽 PR39 基因的腺病毒在小鼠巨噬细胞RAw264.7和小鼠肺部的表达及抗结核分枝杆菌活性.方法:利用携巨噬细胞启动子和抗菌肽 PR39 基因的重组腺病毒感染体外培养的小鼠巨噬细胞RAW264.7和小鼠肺部,采用荧光免疫技术检测PR39 在小鼠肺部表达,并对其抗菌活生进行初步检测.结果:...

Inhibitory effect of macrophage-specific expression of antimicrobial peptide PR-39 on intra-cellular growth of BCG in mouse macrophage cells and lung

Objective：To construct an adenovirus expression vector encoding antimicrobial peptide PR39,and to observe its expression and antimicrobial activity in mouse macrophage RAW264.7 cells and lung.Methods：Recombinant adenovirus vector pAd-PR39 was used to infect mouse macrophage RAW264.7 cells and lungs and its antimicrobial activity against BCG was detected.The expression of PR39 gene in mouse macrophage RAW264.7 cells and lungs was detected by immunocytochemistry.Results：Recombinant adenovirus Ad-PR39 was infected into macrophage RAW264.7 cells and lung for stable or transient expression of PR-39.PR-39 expression in macrophage cells was subsequently confirmed by immunocytochemistry and showed stronger antibacterial ability against BCG than control group.Conclusions：These results demonstrate that macrophage-specific expression of antimicrobial peptide PR-39 in macrophages can inhibit the growth of intracellular BCG and it is a novel and promising approach for the control of refractory intracellular infection.