化学刺激致溃疡性结肠炎兔模型的建立

目的:建立溃疡性结肠炎兔模型并探讨其应用价值.方法:30 只成年健康新西兰兔随机分为 5组,每组 6 只,实验组分别用5乙酸、8乙酸、10乙酸灌肠造模,阳性对照组以三硝基苯磺酸(TN-BS)灌肠造模,空白对照组以生理盐水灌肠,比较各组疾病活动指数(DAI),并进行组织学观察.结果:5乙酸造模组DAI与TNBS对照组无统...

Chemical stimulation induced ulcerative colitis in rabbit models

Objective：To establish a rabbit model of ulcerative colitis.Methods：Thirty adult white rabbits of New Zealand were divided into five groups randomly,each having 6 animals.The experimental groups were treated with 5%,8% and 10% acetic acid to induce the enema modeling.TNBS enema modeling（positive control group） and negative control group were also created for comparison of DAI among these groups.Historical analysis was also performed.Results：The group treated with 5% acetic acid showed no significant difference in DAI comparing with the positive control group（P0.05）,but the group treated with 10% acetic acid group showed significant differences when comparing with the rest 4 groups（P0.05～0.01）.Histological observation revealed that increased intestinal walls,mucosal bleeding,ulcers,and necrosis.Specifically,the 5% acetic acid treated group showed inflammatory,cell infiltration and disappearance of glands,etc.The other 3 groups also showed mucosal necrosis and fall.Conclusions：Topical stimulation of 5% acetic acid can induce ulcer colitis in rabbit model.It＇s also cheap,quick and has good reproducibility.