缺氧预处理对不同鼠龄大鼠海马神经干细胞增殖和分化的影响

目的:探讨缺氧预处理对不同鼠龄大鼠海马神经干细胞(NSCs)增殖和分化的影响.方法:随机选取新生1天(Q1组)、新生7天(Q7组)、新生14天(Q14组)大鼠各6只,分别缺氧2小时.将各组大鼠海马在无血清培养液中,进行原代、单克隆和传代培养;单克隆培养细胞进行巢蛋白(Nestin)免疫荧光染色;传三代细胞诱导分化3d后...

Influence of hypoxia on proliferation and differentiation of neural stem cells from hippocampus of neonate rats

Objective：To explore the influence of hypoxia on proliferation and differentiation of neural stem cells（NSCs） of hippocampus in neonate rats.Methods：Eighteen neonate rats（group Q1,6 rats,1 day after birth;group Q7,6 rats,7 days after birth;group Q14,6 rats,7 days after birth） were exposed to hypoxia status for 2 hours.Then hippocampi of each group were put in the serum-free medium for monoclonal culture or subcultivation.Then,monocloned cells were induced to be differentiated by fetal bovine serum for 3 days,and then nestin positive cells were identified by immunofluorescence.Neuron specific enolase（NSE） positive or glial fibrillary acidic protein（GFAP） positive cells of 3G subcultivated NSCs were calculated.Results：All monoclonal cells were nestin positive;while only parts of the subcultivated cells were NSE positive or GFAP positive.It took 7d,11d and 40d for the monoclonal cells of group Q1,Q7 and Q14 to develop into a diameter of 200μm.The percentage of NSE positive cells was significant higher in Q1 than that in Q14.Conclusions：Hypoxia pretreatment can promote proliferation of hippocampus NSCs and increase the percentage of NSE positive cells more obviously Q1 rats than that in Q 14 rats.