白细胞介素-1B-511基因多态性与牙周炎进展的关系

目的 了解不同程度慢性牙周炎病程的自然发展及其与白细胞介素-1B-511（IL-1B-511）基因多态性的关系。方法 选取慢性牙周炎患者100例，分别在基线、6个月、12个月时检查全口余留牙的临床附着丧失量（AL），每个牙检查6个位点。棉拭子刮取患者颊黏膜脱落细胞，采用聚合酶链反应检测IL-1B-511基因型分布。结果 1年内，100例患者平均AL增加1.43 mm；病情进展缓慢（全口年平均AL增加不超过1.0 mm）的16例，进展快速（全口年平均AL增加超过1.0 mm）的84例；IL-1B-511基因型分布及等位基因分布频率在病情进展缓慢和快速的患者间未发现统计学差异（P>0.05）。基线检查时为轻中度牙周炎的患者在1年的观察期内出现全口年平均AL增加超过1.0 mm的人数多于重度牙周炎患者（P<0.05）；从患牙部位看，两次复查时AL增加均超过2.0 mm的牙位以磨牙为多，多于前牙和前磨牙；且在重度牙周炎患者中更易出现，多于轻中度牙周炎患者（P<0.05）。结论 不同个体牙周炎的进展程度不一致；AL改变与白细胞介素-1基因多态性尚未发现有相关性。

Association of the Progress of Chronic Periodontitis with Interleukin-1B-511 Genetic Polymorphisms

OBJECTIVE: To study the natural progress of different degree chronic periodontitis and its association with IL-1B-511 genetic polymorphisms. METHODS; 100 subjects with chronic periodontitis were selected and examined at baselined and in the 6 month and in 1 year on attatchment loss at 6 sites of each tooth. DNA samples were obtained with buccal swabbing technique and were further analyzed for IL-1B-511 genotype polymorphisms using PCR-RFLP-based method in all subjects. RESULTS: The mean AL increases were 1.43 mm within 1 year. Among 100 subjects, 16 patients with moderate progression (0 mm < AL increase/a year < or = 1.0 mm), 84 patients with rapid progression of periodontal disease (AL increase/ a year > 1.0 mm). There was no significant difference for the distribution and frequency of IL-1B-511 genotype and alleles between the AL increase/ a year > 1.0 mm group and AL increase/a year < or = 1.0 mm group. The progression of periodontal disease (AL increase/a year > 1.0 mm group) was significantly higher in the non-severe chronic periodontitis group than in the severe group (P < 0.05). The percentage of molar was higher as far as the rapid-progress sites (AL increase > 2.0 mm both in the 6th and the 12th months examination) were concerned than that of premolar and anterior (P < 0.05). The number of progressed sites in the severe group was higher than the non-severe chronic periodontitis group (P < 0.05). CONCLUSION: The progress of chronic periodontits varies individually. No specific relationship was found between the progression of chronic periodontitis and IL-1 gene polymorphisms.